PMID- 11860494
OWN - NLM
STAT- MEDLINE
DCOM- 20020412
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 14
IP  - 12
DP  - 2001 Dec
TI  - Estrogen and exercise interact to regulate brain-derived neurotrophic factor mRNA 
      and protein expression in the hippocampus.
PG  - 1992-2002
AB  - We investigated the possibility that estrogen and exercise interact in the 
      hippocampus and regulate brain-derived neurotrophic factor (BDNF), a molecule 
      increasingly recognized for its role in plasticity and neuron function. An 
      important aspect of this study is to examine the effect of different time 
      intervals between estrogen loss and estrogen replacement intervention. We 
      demonstrate that in the intact female rat, physical activity increases 
      hippocampal BDNF mRNA and protein levels. However, the exercise effect on BDNF 
      up-regulation is reduced in the absence of estrogen, in a time-dependent manner. 
      In addition, voluntary activity itself is stimulated by the presence of estrogen. 
      In exercising animals, estrogen deprivation reduced voluntary activity levels, 
      while estrogen replacement restored activity to normal levels. In sedentary 
      animals, estrogen deprivation (ovariectomy) decreased baseline BDNF mRNA and 
      protein, which were restored by estrogen replacement. Despite reduced activity 
      levels in the ovariectomized condition, exercise increased BDNF mRNA levels in 
      the hippocampus after short-term (3 weeks) estrogen deprivation. However, 
      long-term estrogen-deprivation blunted the exercise effect. After 7 weeks of 
      estrogen deprivation, exercise alone no longer affected either BDNF mRNA or 
      protein levels. However, exercise in combination with long-term estrogen 
      replacement increased BDNF protein above the effects of estrogen replacement 
      alone. Interestingly, protein levels across all conditions correlated most 
      closely with mRNA levels in the dentate gyrus, suggesting that expression of mRNA 
      in this hippocampal region may be the major contributor to the hippocampal BDNF 
      protein pool. The interaction of estrogen, physical activity and hippocampal BDNF 
      is likely to be an important issue for maintenance of brain health, plasticity 
      and general well-being, particularly in women.
FAU - Berchtold, N C
AU  - Berchtold NC
AD  - Institute for Brain Aging and Dementia, 1226 Gillespie Neuroscience Facility, 
      University of California, Irvine, CA 92697-4540, USA. nberchto@uci.edu
FAU - Kesslak, J P
AU  - Kesslak JP
FAU - Pike, C J
AU  - Pike CJ
FAU - Adlard, P A
AU  - Adlard PA
FAU - Cotman, C W
AU  - Cotman CW
LA  - eng
GR  - AG 13880/AG/NIA NIH HHS/United States
GR  - AG 15961/AG/NIA NIH HHS/United States
GR  - R01 AG 13411-04/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Estrogens)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Estrogen Replacement Therapy
MH  - Estrogens/blood/*deficiency/pharmacology
MH  - Female
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Hippocampus/drug effects/*metabolism
MH  - Menopause/*metabolism
MH  - Motor Activity/drug effects/physiology
MH  - Neurons/drug effects/metabolism
MH  - Physical Conditioning, Animal/*physiology
MH  - RNA, Messenger/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Up-Regulation/drug effects/physiology
EDAT- 2002/02/28 10:00
MHDA- 2002/04/16 10:01
CRDT- 2002/02/28 10:00
PHST- 2002/02/28 10:00 [pubmed]
PHST- 2002/04/16 10:01 [medline]
PHST- 2002/02/28 10:00 [entrez]
AID - 1825 [pii]
AID - 10.1046/j.0953-816x.2001.01825.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2001 Dec;14(12):1992-2002. doi: 10.1046/j.0953-816x.2001.01825.x.