PMID- 11861498
OWN - NLM
STAT- MEDLINE
DCOM- 20020321
LR  - 20071114
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 143
IP  - 3
DP  - 2002 Mar
TI  - Expression, hormonal regulation, and cyclic variation of chemokines in the rat 
      ovary: key determinants of the intraovarian residence of representatives of the 
      white blood cell series.
PG  - 784-91
AB  - A growing body of evidence suggests that mammalian ovulation bears similarities 
      to local inflammatory reactions. Monocytes/macrophages, eosinophils, and 
      neutrophils are known to infiltrate the area surrounding the dominant follicle 
      before ovulation. Candidate local chemoattractants may include a family of small 
      cytokines, also known as chemokines. In the present study, quantitative RT-PCR 
      was used to initially identify and quantify the chemokines expressed in the 
      preovulatory rat ovary. The chemokines monocyte chemotatic protein 1 (MCP-1), 
      MCP-3, macrophage inflammatory protein 1alpha (MIP-1alpha), MIP-1beta, 
      MIP-1gamma, regulated upon activation normal T cell expressed and secreted, 
      eotaxin, interferon-inducible protein of 10 kDa, growth-regulated oncogene, 
      lymphotactin, and fractalkine were all expressed in the PMSG-primed rat ovary 6 h 
      post human CG. C10, T cell activation gene 3, exodus, exodus-2, cytokine-induced 
      neutrophil chemoattractant-2, MIP-2, and lipopolysaccharide-induced C-X-C were 
      not expressed in the PMSG-primed rat ovary 6 h post human CG. The cyclic 
      variation of the ovary-positive chemokines was also evaluated throughout the 
      course of a superovulated ovarian cycle. Significant preovulatory up-regulation 
      relative to the untreated control state was documented for MCP-1 (18-fold), MCP-3 
      (12-fold), and growth-regulated oncogene (25-fold). In contrast, the preovulatory 
      ovarian expression of eotaxin, fractalkine and regulated upon activation normal T 
      cell expressed and secreted was not increased. These observations suggest that 
      intraovarian chemokines may be responsible for the cyclic intraovarian residence 
      of representatives of the white blood cell series.
FAU - Wong, Kenneth H H
AU  - Wong KH
AD  - Division of Reproductive Sciences, Department of Obstetrics and Gynecology, 
      University of Utah Health Sciences Center, Huntsman Cancer Institute, Salt Lake 
      City, Utah 84112, USA.
FAU - Negishi, Hiroaki
AU  - Negishi H
FAU - Adashi, Eli Y
AU  - Adashi EY
LA  - eng
GR  - HD-30288/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Actins)
RN  - 0 (Chemokines)
RN  - 0 (DNA, Complementary)
RN  - 0 (Estrogens)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Chemokines/*biosynthesis/genetics/physiology
MH  - Chemotaxis, Leukocyte/physiology
MH  - DNA, Complementary/biosynthesis/genetics
MH  - Estrogens/*physiology
MH  - Estrous Cycle/*metabolism
MH  - Female
MH  - Leukocytes/*physiology
MH  - Ovary/*metabolism
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Superovulation/physiology
MH  - Transcription, Genetic
MH  - Up-Regulation/physiology
EDAT- 2002/02/28 10:00
MHDA- 2002/03/22 10:01
CRDT- 2002/02/28 10:00
PHST- 2002/02/28 10:00 [pubmed]
PHST- 2002/03/22 10:01 [medline]
PHST- 2002/02/28 10:00 [entrez]
AID - 10.1210/endo.143.3.8699 [doi]
PST - ppublish
SO  - Endocrinology. 2002 Mar;143(3):784-91. doi: 10.1210/endo.143.3.8699.