PMID- 11861759
OWN - NLM
STAT- MEDLINE
DCOM- 20021230
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 115
IP  - Pt 3
DP  - 2002 Feb 1
TI  - Pax3 induces cell aggregation and regulates phenotypic mesenchymal-epithelial 
      interconversion.
PG  - 517-29
AB  - Paired box-containing transcription factors play fundamental roles in pattern 
      formation during embryonic development of diverse organisms ranging from 
      Drosophila to mammals. Although mutations to Pax3 and other Pax-family genes in 
      both mice and humans result in numerous tissue-specific morphological defects, 
      little is known about the cellular processes that Pax genes regulate. We show 
      that extopic Pax3 expression in two distinct phenotypically mesenchymal mammalian 
      cell lines induces the formation of multi-layered condensed cell aggregates with 
      epithelial characteristics. For one of these lines, we showed further that 
      Pax3-induced cell aggregation is accompanied by specific morphological changes, 
      including a significant reduction in cell size, altered cell shape and dramatic 
      alterations to both membrane and cytoskeleton architecture. In addition to 
      mediating a phenotypic mesenchymal-to-epithelial transition, Pax3 also 
      establishes the conditions in these cells for a subsequent hepatocyte growth 
      factor/scatter factor (HGF/SF)-induced phenotypic epithelial-to-mesenchymal 
      transition. Thus, our data show a novel morphogenetic activity for Pax3 which, 
      when absent in vivo, is predicted to give rise to the observed structural defects 
      in somites and the neural tube during embryonic development.
FAU - Wiggan, O'Neil
AU  - Wiggan O
AD  - Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, 
      University of Toronto, Toronto, Ontario, M5S 1A8 Canada.
FAU - Fadel, Marc P
AU  - Fadel MP
FAU - Hamel, Paul A
AU  - Hamel PA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Actins)
RN  - 0 (Cadherins)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (PAX3 Transcription Factor)
RN  - 0 (PAX3 protein, human)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (Phosphoproteins)
RN  - 0 (TJP1 protein, human)
RN  - 0 (Tjp1 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 138016-91-8 (Pax3 protein, mouse)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Actins/metabolism
MH  - Adenoviridae/genetics/metabolism
MH  - Animals
MH  - COS Cells
MH  - Cadherins/metabolism
MH  - Cell Aggregation/*physiology
MH  - Cell Polarity
MH  - Cell Size
MH  - Culture Media, Conditioned
MH  - Cytoskeletal Proteins/metabolism
MH  - Cytoskeleton/metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Epithelium/*metabolism
MH  - Genes, Reporter
MH  - Hepatocyte Growth Factor/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Membrane Proteins/metabolism
MH  - Mesoderm/*metabolism
MH  - Microtubules/metabolism
MH  - Osteosarcoma
MH  - PAX3 Transcription Factor
MH  - Paired Box Transcription Factors
MH  - Phenotype
MH  - Phosphoproteins/metabolism
MH  - Proto-Oncogene Proteins c-met/metabolism
MH  - Rhabdomyosarcoma
MH  - Transcription Factors/*metabolism
MH  - Tumor Cells, Cultured
MH  - Zonula Occludens-1 Protein
EDAT- 2002/02/28 10:00
MHDA- 2002/12/31 04:00
CRDT- 2002/02/28 10:00
PHST- 2002/02/28 10:00 [pubmed]
PHST- 2002/12/31 04:00 [medline]
PHST- 2002/02/28 10:00 [entrez]
AID - 10.1242/jcs.115.3.517 [doi]
PST - ppublish
SO  - J Cell Sci. 2002 Feb 1;115(Pt 3):517-29. doi: 10.1242/jcs.115.3.517.