PMID- 11861913
OWN - NLM
STAT- MEDLINE
DCOM- 20020311
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 30
IP  - 5
DP  - 2002 Mar 1
TI  - The 3'-untranslated regions of cytoskeletal muscle mRNAs inhibit translation by 
      activating the double-stranded RNA-dependent protein kinase PKR.
PG  - 1205-12
AB  - Cytoskeletal proteins are associated with actin in the microfilaments and have a 
      major role in microfilament assembly and function. The expression of some of 
      these proteins has been implicated in cell growth and transformation. 
      Specifically, the 3'-untranslated regions (3'-UTRs) of tropomyosin, troponin and 
      cardiac actin can induce muscle cell differentiation and appear to function as 
      tumor suppressors. These RNA sequences are predicted to fold to form secondary 
      structures with extended stretches of duplex. We show that the 3'-UTRs of the 
      cytoskeletal mRNAs interact with the RNA-binding domain of the RNA-activated 
      protein kinase PKR. Correspondingly, these RNAs activate PKR in vitro and inhibit 
      globin translation in the rabbit reticulocyte lysate translation system. These 
      data are consistent with a mechanism whereby PKR mediates the differentiation- 
      and tumor-related actions of the cytoskeletal 3'-UTR sequences.
FAU - Nussbaum, Jean M
AU  - Nussbaum JM
AD  - Department of Biochemistry and Molecular Biology, New Jersey Medical School, 
      UMDNJ, 185 South Orange Avenue, Newark, NJ 07103-2714, USA.
FAU - Gunnery, Shobha
AU  - Gunnery S
FAU - Mathews, Michael B
AU  - Mathews MB
LA  - eng
GR  - R01 AI034552/AI/NIAID NIH HHS/United States
GR  - AI34552/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Actins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Tropomyosin)
RN  - 0 (Troponin)
RN  - EC 2.7.11.1 (eIF-2 Kinase)
SB  - IM
MH  - 3' Untranslated Regions/metabolism/*pharmacology
MH  - Actins/genetics
MH  - Animals
MH  - Cytoskeletal Proteins/*genetics
MH  - Electrophoretic Mobility Shift Assay
MH  - Enzyme Activation
MH  - Muscle Proteins/*genetics
MH  - Nucleic Acid Conformation
MH  - *Protein Biosynthesis
MH  - Rabbits
MH  - Reticulocytes/metabolism
MH  - Tropomyosin/genetics
MH  - Troponin/genetics
MH  - eIF-2 Kinase/*metabolism
PMC - PMC101232
EDAT- 2002/02/28 10:00
MHDA- 2002/03/12 10:01
PMCR- 2002/03/01
CRDT- 2002/02/28 10:00
PHST- 2002/02/28 10:00 [pubmed]
PHST- 2002/03/12 10:01 [medline]
PHST- 2002/02/28 10:00 [entrez]
PHST- 2002/03/01 00:00 [pmc-release]
AID - gkf192 [pii]
AID - 10.1093/nar/30.5.1205 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2002 Mar 1;30(5):1205-12. doi: 10.1093/nar/30.5.1205.