PMID- 11864396
OWN - NLM
STAT- MEDLINE
DCOM- 20031006
LR  - 20061115
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 37
IP  - 2
DP  - 2001 Mar
TI  - [Immunohistochemical and clinical studies on sub-corneal flap epithelial 
      implantation accompanied by flap melting after excimer laser in situ 
      keratomileusis].
PG  - 84-6
AB  - OBJECTIVE: To study the immunohistochemical and clinical characteristics of the 
      corneal epithelial cells implanted under the flap accompanied by the flap melting 
      after excimer laser in situ keratomileusis (LASIK) and the method of management. 
      METHODS: In eight eyes (In 4 eyes a part of the flap melted.) with corneal 
      epithelial cells implanted under the flap after LASIK, the corneal flap was torn 
      again. The implanted tissue was removed. After washing between the interfaces, 
      the flap was repositioned. The implanted tissue was immunohistochemically stained 
      by using four kinds of monoclonal antibodies and using terminal 
      deoxyribonucleotide transferase-mediated dUTP nick end labeling (TUNEL) method. 
      RESULTS: Early in the course, the disorder appeared on the temporal side, 
      expanded and thickened. The defective periphery of the corneal flap presented as 
      a partial lunar eclipse. The melting process was self-limited in 3 months. The 
      result was good after the implanted tissue was removed. The implanted material 
      was stained by using HE, immunohistochemical and TUNEL methods. Some vesicle-like 
      degeneration in the implanted layer was found. There was lysozyme in the 
      vesicles, and the cells of implanted layer possessed a trend of apoptosis. All 
      the cells were dead in the vesicles. CONCLUSIONS: The progress of corneal 
      epithelial implantation under corneal flap after LASIK may include 3 periods: 
      epithelial implantation, corneal flap melting and self-limitation. Early in the 
      course, the implanted material presented as a thin film. Afterwards, it becomes a 
      non-structural material. Possibly the melting of the corneal flap is caused by 
      apoptosis. Therefore, the implanted epithelium should be removed early.
FAU - Du, Z
AU  - Du Z
AD  - Ophthalmic Center, The Second Affiliated Hospital, Chongqing University of 
      Medical Sciences, Chongqing 400010, China.
FAU - Guo, H
AU  - Guo H
FAU - Zheng, Q
AU  - Zheng Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Adult
MH  - Apoptosis
MH  - Corneal Opacity/pathology/*surgery
MH  - Cyclin D1/analysis
MH  - Epithelium, Corneal/chemistry/pathology/*transplantation
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Keratomileusis, Laser In Situ/*methods
MH  - Male
MH  - Muramidase/analysis
MH  - Proto-Oncogene Proteins c-bcl-2/analysis
MH  - *Surgical Flaps
MH  - Visual Acuity
EDAT- 2002/02/28 10:00
MHDA- 2003/10/08 05:00
CRDT- 2002/02/28 10:00
PHST- 2002/02/28 10:00 [pubmed]
PHST- 2003/10/08 05:00 [medline]
PHST- 2002/02/28 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 2001 Mar;37(2):84-6.