PMID- 11865089 OWN - NLM STAT- MEDLINE DCOM- 20020709 LR - 20220318 IS - 0931-0509 (Print) IS - 0931-0509 (Linking) VI - 17 IP - 3 DP - 2002 Mar TI - Circulating levels of ICAM-1, VCAM-1, and MCP-1 are increased in haemodialysis patients: association with inflammation, dyslipidaemia, and vascular events. PG - 435-41 AB - BACKGROUND: Increased levels of circulating adhesion molecules and chemokines have been reported in haemodialysis (HD) patients but the influence of the HD membranes on their secretion, as well as their pathophysiological implications, remains largely unknown. METHODS: Circulating levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and monocyte chemoattractant protein-1 (MCP-1) were measured by immunosorbent assay (ELISA) in 81 HD patients (45 male, mean age 57+/-13 years) and 35 normal subjects. All patients had been stabilized on renal replacement therapy for >3 months and were free of active infection. Thirty-three patients (40.7%) were routinely dialysed with modified cellulose membranes and 48 patients (59.3%) were dialysed with polysulfone membranes. Blood samples were taken directly from the arteriovenous fistula immediately before and at the end of a routine HD session. RESULTS: Pre-dialysis levels were significantly elevated in HD patients compared with controls (ICAM-1 515+/-177 vs 238+/-664 ng/ml, P<0.0001; VCAM-1 2107+/-648 vs 1012+/-115 ng/ml, P<0.0001; MCP-1 427+/-148 vs 125+/-42 pg/ml, P<0.0001). The HD session resulted in a significant increase in the levels of all three molecules measured (515+/-177 vs 679+/-187 ng/ml, P<0.0001; 2107+/-648 vs 2662+/-800 ng/ml, P<0.0001; 427+/-148 vs 567+/-153 pg/ml, P<0.0001, respectively). There was no difference in pre- or post-dialysis levels of the above molecules between patients routinely dialysed with either modified cellulose or polysulfone membranes. MCP-1 levels had a positive correlation with ICAM-1 levels (r=0.41, P<0.0005). VCAM-1 levels had a negative correlation with HDL levels (r=-0.30, P<0.01) and were significantly elevated in patients with HDL <35 mg/dl compared with patients with HDL > or = 35 mg/dl (2300+/-606 vs 1890+/-633 ng/ml, P<0.005). Log-transformed exact C-reactive protein (CRP) values were significantly correlated with ICAM-1 and VCAM-1 levels (r=0.41, P<0.005 and r=0.43, P<0.005, respectively). In addition, compared with patients with normal CRP values, patients with elevated CRP had significantly increased levels of ICAM-1 (466+/-166 vs 580+/-172 ng/ml, P<0.005). Patients with cardiovascular, cerebrovascular, or peripheral vascular diseases had significantly increased serum CRP and ICAM-1 levels compared with patients with no evidence of vascular disease (19.2+/-12.9 vs 7.9+/-11.8 mg/l, P<0.001 and 608+/-189 vs 474+/-155 ng/ml, P<0.005 respectively). CONCLUSIONS: Serum levels of ICAM-1, VCAM-1, and MCP-1 are increased in HD patients and probably result from either inadequate clearance or enhanced synthesis and release. HD session resulted in a significant increase of the above molecule levels but the exact mechanism(s) responsible for these alterations are yet to be fully elucidated. Increased levels of adhesion molecules are associated with inflammation, dyslipidaemia, and cardiovascular events. However, the potential link between these processes and its clinical significance warrants further investigation. FAU - Papayianni, Aikaterini AU - Papayianni A AD - Department of Nephrology, Hippokration General Hospital, Thessaloniki, Greece. aikpapag@otenet.gr FAU - Alexopoulos, Efstathios AU - Alexopoulos E FAU - Giamalis, Panagiotis AU - Giamalis P FAU - Gionanlis, Lazaros AU - Gionanlis L FAU - Belechri, Anna-Maria AU - Belechri AM FAU - Koukoudis, Paraschos AU - Koukoudis P FAU - Memmos, Dimitrios AU - Memmos D LA - eng PT - Journal Article PL - England TA - Nephrol Dial Transplant JT - Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association JID - 8706402 RN - 0 (Chemokine CCL2) RN - 0 (Vascular Cell Adhesion Molecule-1) RN - 126547-89-5 (Intercellular Adhesion Molecule-1) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Cardiovascular Diseases/blood/etiology MH - Case-Control Studies MH - Chemokine CCL2/*blood MH - Female MH - Humans MH - Hyperlipidemias/blood/etiology MH - Inflammation/blood/etiology MH - Intercellular Adhesion Molecule-1/*blood MH - Male MH - Middle Aged MH - Renal Dialysis/*adverse effects MH - Uremia/blood/complications/therapy MH - Vascular Cell Adhesion Molecule-1/*blood EDAT- 2002/02/28 10:00 MHDA- 2002/07/10 10:01 CRDT- 2002/02/28 10:00 PHST- 2002/02/28 10:00 [pubmed] PHST- 2002/07/10 10:01 [medline] PHST- 2002/02/28 10:00 [entrez] AID - 10.1093/ndt/17.3.435 [doi] PST - ppublish SO - Nephrol Dial Transplant. 2002 Mar;17(3):435-41. doi: 10.1093/ndt/17.3.435.