PMID- 11865298
OWN - NLM
STAT- MEDLINE
DCOM- 20020423
LR  - 20191105
IS  - 0916-4804 (Print)
IS  - 0916-4804 (Linking)
VI  - 43
IP  - 1
DP  - 2002
TI  - Detection of the gp43 gene and (1-3)-beta-D-glucan of Paracoccidioides 
      brasiliensis in the blood of experimentally infected mice.
PG  - 29-35
AB  - Paracoccidioidomycosis (PCM) is a deep mycosis caused by the thermo-dependent 
      dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American 
      countries. Diagnosis of PCM is sometimes difficult outside the endemic countries, 
      thus a rapid and conclusive method for diagnosis of PCM has been anticipated. We 
      compared the sensitivities of a nested PCR method for detecting the gp43 gene and 
      a commercial kit for detecting (1-3)-beta-D-glucan in the blood of experimentally 
      infected mice. Blood samples were collected from mice at 0 (soon after 
      inoculation), 6, 12, 24, 48, and 72 hours and 5, 7, 10, 14, 17, 21, 24, 28 and 56 
      days after the intravenous inoculation of 10(6) yeast cells of P. brasiliensis, 
      and were separated into clots and plasma. The (1-3)-beta-D-glucan detection kit 
      in the plasma showed positive reactions in some samples within 7 days and 28 and 
      56 days after infections. In contrast, the PCR method was more sensitive than the 
      (1-3)-beta-D-glucan detection kit throughout the observation period. The clot 
      samples yielded more sensitive PCR-results than did the plasma samples. Although 
      24 hours is required for the PCR detection, it was confirmed to provide an 
      accurate diagnosis of PCM.
FAU - Nakagawa Itano, Eiko
AU  - Nakagawa Itano E
AD  - Universidade Estadual de Londrina, CCB-PAT-Campus Universitario, CEP: 86051-970, 
      Londrina, Parana, Brazil.
FAU - Uno, Jun
AU  - Uno J
FAU - Sano, Ayako
AU  - Sano A
FAU - Yarita, Kyoko
AU  - Yarita K
FAU - Kamei, Katsuhiko
AU  - Kamei K
FAU - Miyaji, Makoto
AU  - Miyaji M
FAU - Nishimura, Kazuko
AU  - Nishimura K
FAU - Mikami, Yuzuru
AU  - Mikami Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Nihon Ishinkin Gakkai Zasshi
JT  - Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology
JID - 9425640
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Biomarkers)
RN  - 0 (Diphosphates)
RN  - 0 (Drug Combinations)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glucans)
RN  - 0 (Glycoproteins)
RN  - 0 (Nitrates)
RN  - 0 (Polyethylenes)
RN  - 0 (Sensitive Tartar Control Dentifrice)
RN  - 0 (beta-Glucans)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - 9051-97-2 (beta-1,3-glucan)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Antigens, Fungal
MH  - Biomarkers/blood
MH  - Child
MH  - Diphosphates
MH  - Disease Models, Animal
MH  - Drug Combinations
MH  - Female
MH  - *Fungal Proteins
MH  - *Genes, Fungal
MH  - Glucans/*blood
MH  - Glycoproteins/*genetics
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Middle Aged
MH  - Nitrates
MH  - Paracoccidioides/*genetics
MH  - Paracoccidioidomycosis/*diagnosis
MH  - Polyethylenes
MH  - Polymerase Chain Reaction/methods
MH  - Sodium Fluoride
MH  - *beta-Glucans
EDAT- 2002/02/28 10:00
MHDA- 2002/04/24 10:01
CRDT- 2002/02/28 10:00
PHST- 2002/02/28 10:00 [pubmed]
PHST- 2002/04/24 10:01 [medline]
PHST- 2002/02/28 10:00 [entrez]
AID - 10.3314/jjmm.43.29 [doi]
PST - ppublish
SO  - Nihon Ishinkin Gakkai Zasshi. 2002;43(1):29-35. doi: 10.3314/jjmm.43.29.