PMID- 11866891
OWN - NLM
STAT- MEDLINE
DCOM- 20040924
LR  - 20160818
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 29
IP  - 1
DP  - 2000 Feb
TI  - [Malignant transformation of mouse embryonic fibroblast induced by mitochondrial 
      DNA fragments].
PG  - 39-42
AB  - OBJECTIVE: To investigate the malignant transforming effect and mechanism of 
      mitochondrial DNA (mtDNA) fragments. METHODS: Tumorigenicity of mtDNA-transformed 
      mouse embryonic fibroblast (NIH3T3) in nude mice was studied using transgenic 
      techniques. Transformed tumors were detected by pathological examination and 
      hybridization signals of mtDNA probe were analyzed by fluorescence in situ 
      hybridization (FISH) techniques. RESULTS: Hybridization signals were observed on 
      the nuclei of 18% - 20% NIH 3T3 cells 1 week after mtDNA fragments transforming. 
      Tumor from mtDNA-transformed NIH 3T3 cells was developed in all 8 nude mice (8/8) 
      respectively 2 weeks after the transformation. The pathological characteristics 
      of the tumors developed were similar to that of fibrosarcoma. CONCLUSIONS: 
      Auto-integration of mtDNA fragments into nuclear genome is a new factor involved 
      in carcinogenesis.
FAU - Hu, Y
AU  - Hu Y
AD  - PLA Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical 
      University, Chongqing 400037, China. floride@online.sh.cn
FAU - Qian, G
AU  - Qian G
FAU - Mao, B
AU  - Mao B
FAU - Xiao, T
AU  - Xiao T
FAU - Li, Y
AU  - Li Y
FAU - Cao, S
AU  - Cao S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Animals
MH  - *Cell Transformation, Neoplastic
MH  - DNA, Mitochondrial/*genetics/isolation & purification
MH  - Fibroblasts/*pathology
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - NIH 3T3 Cells
MH  - Neoplasm Transplantation
MH  - Neoplasms, Experimental/genetics/*pathology
MH  - Peptide Fragments/genetics
EDAT- 2002/02/28 10:00
MHDA- 2004/09/25 05:00
CRDT- 2002/02/28 10:00
PHST- 2002/02/28 10:00 [pubmed]
PHST- 2004/09/25 05:00 [medline]
PHST- 2002/02/28 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2000 Feb;29(1):39-42.