PMID- 11868823
OWN - NLM
STAT- MEDLINE
DCOM- 20020903
LR  - 20191025
IS  - 0190-2148 (Print)
IS  - 0190-2148 (Linking)
VI  - 28
IP  - 2
DP  - 2002 Mar
TI  - Human bronchial epithelium expresses interleukin-9 receptors and releases 
      neutrophil chemotactic factor.
PG  - 123-39
AB  - Growing evidence obtained from human genomic analysis and antigen-challenged 
      transgenic mice suggests that interleukin-9 (IL-9) is a candidate factor in 
      immunoglobulin E (IgE) production and thus is thought to be associated with 
      bronchial inflammation and bronchial hyperresponsiveness (BHR). To evaluate the 
      expression of the IL-9 receptor and its effect on the IL-9 human bronchial cell 
      line BEAS-2B cells, reverse transcriptase-polymerase chain reaction (RT-PCR), 
      immunohistochemical investigation, and chemotaxis assay were performed. The 
      components of the IL-9 receptor, consisting of IL-9 receptor alpha (CD129) and 
      IL-2 receptory ((1)132), were expressed on BEAS-2B cells as determined by RT-PCR 
      and flow cytometry. BEAS-2B cells exposed to IL-9 released neutrophil chemotactic 
      activity (NCA) in a time- and dose-dependent manner, and the presence of 
      granulocyte colony-stimulating factor (G-CSF) was also detected. This factor is 
      primarily involved in NCA for the measurement of cytokines and in the inhibition 
      assay of neutrophil chemotaxis. These findings suggest that bronchial epithelial 
      cells may express IL-9 receptors, and that IL-9 may induce airway inflammation 
      through the release of G-CSF from bronchial epithelial cells.
FAU - Tsukadaira, Akihiro
AU  - Tsukadaira A
AD  - First Department of Internal Medicine, Shinshu University School of Medicine, 
      Matsumoto, Japan.
FAU - Okubo, Yoshio
AU  - Okubo Y
FAU - Koyama, Sekiya
AU  - Koyama S
FAU - Sato, Etsuro
AU  - Sato E
FAU - Nagase, Hisashi
AU  - Nagase H
FAU - Agematsu, Kazunaga
AU  - Agematsu K
FAU - Nakazawa, Koh
AU  - Nakazawa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Exp Lung Res
JT  - Experimental lung research
JID - 8004944
RN  - 0 (Antibodies)
RN  - 0 (IL9R protein, human)
RN  - 0 (Interleukin-8)
RN  - 0 (Interleukin-9)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-2)
RN  - 0 (Receptors, Interleukin-9)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Antibodies/pharmacology
MH  - Bronchi/cytology/immunology
MH  - Cell Line
MH  - Gene Expression/drug effects/immunology
MH  - Granulocyte Colony-Stimulating Factor/immunology
MH  - Humans
MH  - Interleukin-8/immunology/*metabolism
MH  - Interleukin-9/metabolism/pharmacology
MH  - Neutrophils/cytology
MH  - RNA, Messenger/analysis
MH  - Receptors, Interleukin/*genetics
MH  - Receptors, Interleukin-2/genetics
MH  - Receptors, Interleukin-9
MH  - Respiratory Mucosa/cytology/immunology/*metabolism
EDAT- 2002/03/01 10:00
MHDA- 2002/09/11 10:01
CRDT- 2002/03/01 10:00
PHST- 2002/03/01 10:00 [pubmed]
PHST- 2002/09/11 10:01 [medline]
PHST- 2002/03/01 10:00 [entrez]
AID - 10.1080/019021402753462040 [doi]
PST - ppublish
SO  - Exp Lung Res. 2002 Mar;28(2):123-39. doi: 10.1080/019021402753462040.