PMID- 11869081
OWN - NLM
STAT- MEDLINE
DCOM- 20020408
LR  - 20161018
IS  - 1063-4584 (Print)
IS  - 1063-4584 (Linking)
VI  - 10
IP  - 3
DP  - 2002 Mar
TI  - The prevalence of nitric oxide in apoptotic chondrocytes of osteoarthritis.
PG  - 207-11
AB  - OBJECTIVE: Apoptosis appears to be a significant mechanism of chondrocyte death 
      in osteoarthritis (OA). There is increasing evidence that nitric oxide (NO) may 
      be the inducing signal for apoptosis, but no study has definitively shown an 
      association between the two in vivo. In this study, sections of osteoarthritic 
      cartilage were double stained for the presence of apoptosis and NO to test the 
      hypothesis that NO is the inducer of apoptosis in arthritis. DESIGN: Sections of 
      osteoarthritic cartilage obtained during total knee arthroplasty were stained for 
      apoptosis with terminal transferase-mediated dUTP nick end labeling (TUNEL). The 
      sections were then stained for nitrotyrosine (a marker of NO production) by 
      immunohistochemistry. The prevalence of NO in cells positive for apoptosis and in 
      cells negative for apoptosis was determined by fluorescent microscopy. RESULTS: 
      The prevalence of NO in apoptotic cells was no different than in non-apoptotic 
      cells, suggesting NO is not the initiating signal for apoptosis in vivo. 
      CONCLUSIONS: The precipitating cause for apoptosis in arthritic chondrocytes has 
      not yet been determined. The data from this study fail to support NO as the 
      direct initiating signal. NO synthase inhibitors may still be useful in the 
      treatment of OA by blocking the catabolic activities of NO.
CI  - Copyright 2002 OsteoArthritis Research Society International.
FAU - Greisberg, J
AU  - Greisberg J
AD  - Brown University/Rhode Island Hospital Department of Orthopaedics, Providence, 
      Rhode Island 02905, USA.
FAU - Bliss, M
AU  - Bliss M
FAU - Terek, R
AU  - Terek R
LA  - eng
PT  - Journal Article
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Apoptosis/*physiology
MH  - Cartilage/chemistry
MH  - Chondrocytes/*metabolism
MH  - Humans
MH  - Nitric Oxide/*metabolism
MH  - Osteoarthritis, Knee/*pathology
EDAT- 2002/03/01 10:00
MHDA- 2002/04/09 10:01
CRDT- 2002/03/01 10:00
PHST- 2002/03/01 10:00 [pubmed]
PHST- 2002/04/09 10:01 [medline]
PHST- 2002/03/01 10:00 [entrez]
AID - S1063458401904972 [pii]
AID - 10.1053/joca.2001.0497 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2002 Mar;10(3):207-11. doi: 10.1053/joca.2001.0497.