PMID- 11869475
OWN - NLM
STAT- MEDLINE
DCOM- 20020403
LR  - 20131121
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 78
IP  - 3
DP  - 2002 Mar
TI  - Chromosome aberrations detected by FISH and correlation with cell survival after 
      irradiation at various dose-rates and after bromodeoxyuridine radiosensitization.
PG  - 203-10
AB  - PURPOSE: To determine whether measurement of chromosome aberrations by 
      fluorescence in situ hybridization (FISH) predicts cell survival after 
      irradiation at different dose-rates and after radiosensitization by 
      bromodeoxyurdine (BrdU) in a lung carcinoma cell line. MATERIALS AND METHODS: The 
      human lung carcinoma cell line SW1573 was irradiated at high dose-rate (HDR: 0.8 
      Gy min-1) or at pulsed low dose-rate (p-LDR: average dose-rate 1 Gy h-1) with or 
      without radiosensitization by bromodeoxyuridine (BrdU). Cell survival was 
      determined by clonogenic assay. Chromosome aberrations (colour junctions) were 
      measured by whole-chromosome FISH of chromosome 2 and 18 and were scored 
      according to the PAINT method. RESULTS: Clear radiosensitization by BrdU was 
      observed both after HDR and p-LDR irradiation. Chromosome 18 was more 
      radiosensitive than chromosome 2. There was a good correlation between induction 
      of colour junctions and cell survival both after HDR and p-LDR irradiation and 
      after radiosensitization by BrdU. CONCLUSIONS: Determination of chromosome 
      aberrations by FISH can predict cell survival after different dose-rates and 
      after radiosensitization by BrdU
FAU - Castro Kreder, N
AU  - Castro Kreder N
AD  - Academic Medical Center, University of Amsterdam, Department of Radiotherapy, PO 
      BOX 22700, 1100 DE Amsterdam, The Netherlands. N.Castro@amc.uva.nl
FAU - Van Bree, C
AU  - Van Bree C
FAU - Franken, N A P
AU  - Franken NA
FAU - Haveman, J
AU  - Haveman J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Bromodeoxyuridine/pharmacology
MH  - Cell Survival/drug effects/*genetics/*radiation effects
MH  - Chromosome Aberrations/drug effects/*radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Radiation Tolerance/drug effects
MH  - Tumor Cells, Cultured
MH  - Tumor Stem Cell Assay
EDAT- 2002/03/01 10:00
MHDA- 2002/04/04 10:01
CRDT- 2002/03/01 10:00
PHST- 2002/03/01 10:00 [pubmed]
PHST- 2002/04/04 10:01 [medline]
PHST- 2002/03/01 10:00 [entrez]
AID - 10.1080/09553000110104074 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2002 Mar;78(3):203-10. doi: 10.1080/09553000110104074.