PMID- 11870602
OWN - NLM
STAT- MEDLINE
DCOM- 20020822
LR  - 20190922
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 78
IP  - 2
DP  - 2002 Apr 20
TI  - Successional development of sulfate-reducing bacterial populations and their 
      activities in an activated sludge immobilized agar gel film.
PG  - 119-30
AB  - A combination of fluorescence in situ hybridization (FISH), microprofiles, and 
      denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rDNA 
      fragments followed by hybridization analysis with specific probes was applied to 
      investigate successional development of sulfate-reducing bacteria (SRB) community 
      structure and in situ sulfide production activity within an activated sludge 
      immobilized agar gel film. In this model biofilm system, since biases arising 
      from biofilm heterogeneity can be ignored, the population dynamics of SRB in the 
      agar gel is directly related to physiological capability and in situ activity of 
      SRB. Microelectrode measurements showed that an anoxic zone was already developed 
      at the beginning (0 day), a first sulfide production of 0.054 mumol H2S m(-2) x 
      s(-1) was detected during the first week, and the rate increased gradually to 
      0.221 mumol H2S m(-2) x s(-1) in the fifth week. The most active sulfide 
      production zone moved upward to the chemocline and intensified with time to form 
      a narrow zone with high volumetric sulfide production rates. This result 
      coincided with the shift of the spatial distributions of SRB populations 
      determined by FISH. In situ hybridization with probe SRB385 for mainly general 
      SRB of the delta Proteobacteria plus some gram-positive bacteria and probe 660 
      for Desulfobulbus indicated that the most abundant populations of SRB were 
      primarily restricted to near the oxic/anoxic interface (chemocline). A close 
      observation of the development of the vertical distributions of SRB populations 
      revealed that the cell numbers of Desulfobulbus tripled (from 0.5 x 10(8) to 1.5 
      x 10(8) cells cm(-3)) near the oxic/anoxic interface. Similar growth (from 1.0 
      x10(8) to 4.5 x 10(8) cells cm(-3)) of Desulfovibrio-like SRB that hybridized 
      with probe SRB385 was observed. PCR-DGGE followed by hybridization analysis 
      revealed that one Desulfobulbus strain was detected from the beginning, and 
      another strain appeared after 1 week, coinciding with the first detected sulfide 
      production. In addition, three strains hybridizing with probe 687 (possibly 
      Desulfovibrio) were also dominant SRB in the agar gel.
CI  - Copyright 2002 Wiley Periodicals, Inc.
FAU - Okabe, Satoshi
AU  - Okabe S
AD  - Department of Urban and Environmental Engineering, Graduate School of 
      Engineering, Hokkaido University, Sapporo 060-8628, Japan. 
      sokabe@eng.hokudai.ac.jp
FAU - Santegoeds, Cecilia M
AU  - Santegoeds CM
FAU - Watanabe, Yoshimasa
AU  - Watanabe Y
FAU - de Beer, Dirk
AU  - de Beer D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 0 (Sulfates)
RN  - 0 (Sulfides)
RN  - 9002-18-0 (Agar)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Agar/chemistry
MH  - *Biofilms
MH  - Electrophoresis/methods
MH  - Microelectrodes
MH  - Oligonucleotide Array Sequence Analysis/methods
MH  - Oxidation-Reduction
MH  - Oxygen/analysis/metabolism
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
MH  - Sulfates/analysis/*metabolism
MH  - Sulfides/analysis/metabolism
MH  - Sulfur-Reducing Bacteria/*classification/*physiology
EDAT- 2002/03/01 10:00
MHDA- 2002/08/23 10:01
CRDT- 2002/03/01 10:00
PHST- 2002/03/01 10:00 [pubmed]
PHST- 2002/08/23 10:01 [medline]
PHST- 2002/03/01 10:00 [entrez]
AID - 10.1002/bit.10240 [pii]
AID - 10.1002/bit.10240 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2002 Apr 20;78(2):119-30. doi: 10.1002/bit.10240.