PMID- 11872235
OWN - NLM
STAT- MEDLINE
DCOM- 20020809
LR  - 20190906
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 63
IP  - 3
DP  - 2002 Mar
TI  - Functional versus structural matching: can the CTLp test be replaced by HLA 
      allele typing?
PG  - 176-84
AB  - Human leukocyte antigen (HLA) incompatibilities are the most important 
      immunological barriers to bone marrow transplant success when using unrelated 
      donors. Until recently, standards for donor selection included serological 
      methods for HLA class I antigens and DNA-based typing for HLA class II alleles. 
      In our center cytotoxic T-lymphocyte precursor (CTLp) assays have been an 
      integrated part of the search selection procedure as well. More recently, 
      DNA-based typing for HLA class I became available. This allowed us to determine 
      the correlation of CTLp frequencies directed against incompatibilities at the 
      HLA-A, -B, and -C locus in 211 donor-recipient pairs. HLA class I 
      incompatibilities are significantly (p < 0.001) associated with high CTLp 
      frequencies. Exceptions did occur, high CTLp frequencies are seen in 14% of the 
      HLA-A, -B, and -C allele matched pairs, whereas in 7% of the pairs mismatched for 
      HLA-A or -B a low CTLp frequency occurred. The successful outcome of transplants 
      performed in the latter cases suggest that the CTLp test can be used as a tool to 
      detect permissible mismatches when no fully matched donor is available. The 
      influence of HLA-C mismatches on the CTLp outcome was less clear. Although in the 
      majority of mismatched pairs (64%) the CTLp frequency was high, in 36% of the 
      pairs the CTLp frequency was low. Analysis of HLA amino acid sequences was 
      performed on the HLA-C allele mismatched group. An amino acid difference was 
      always found at five polymorphic positions 97, 99, 113, 114, and 116 situated at 
      the peptide binding groove in the high CTLp frequency group, whereas in the low 
      CTLp frequency group this was observed in only 9 of 17 combinations (p < 0.001). 
      However, this is mainly due to Cw*0303-0304 mismatches. In conclusion, although 
      there is a highly significant correlation between the outcome of the CTLp 
      frequency test and HLA allele class I typing, exceptions occur. It is unclear 
      whether they are all clinically relevant but they certainly provide additional 
      insight in allograft recognition.
FAU - Oudshoorn, M
AU  - Oudshoorn M
AD  - Europdonor Foundation, Leiden, The Netherlands.
FAU - Doxiadis, I I N
AU  - Doxiadis II
FAU - van den Berg-Loonen, P M
AU  - van den Berg-Loonen PM
FAU - Voorter, C E M
AU  - Voorter CE
FAU - Verduyn, W
AU  - Verduyn W
FAU - Claas, F H J
AU  - Claas FH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Child
MH  - Graft vs Host Reaction/immunology
MH  - HLA Antigens/*genetics/immunology
MH  - HLA-A Antigens/genetics/immunology
MH  - HLA-B Antigens/genetics/immunology
MH  - HLA-C Antigens/genetics/immunology
MH  - *Histocompatibility Testing
MH  - Humans
MH  - T-Lymphocyte Subsets/*classification
MH  - T-Lymphocytes, Cytotoxic/*classification
EDAT- 2002/03/02 10:00
MHDA- 2002/08/10 10:01
CRDT- 2002/03/02 10:00
PHST- 2002/03/02 10:00 [pubmed]
PHST- 2002/08/10 10:01 [medline]
PHST- 2002/03/02 10:00 [entrez]
AID - S0198885901003846 [pii]
AID - 10.1016/s0198-8859(01)00384-6 [doi]
PST - ppublish
SO  - Hum Immunol. 2002 Mar;63(3):176-84. doi: 10.1016/s0198-8859(01)00384-6.