PMID- 11873816
OWN - NLM
STAT- MEDLINE
DCOM- 20020618
LR  - 20061115
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 33
IP  - 1
DP  - 2002 Jan-Feb
TI  - Reliable ELISAs showing differences between resistant and susceptible lines in 
      hens orally inoculated with Salmonella Enteritidis.
PG  - 23-33
AB  - Reliable ELISAs were investigated with the aim to select hen lines resistant to 
      Salmonella Enteritidis and producing high levels of antibodies. In the first 
      experiment, the relation between the humoral response and the bacteriological 
      results was assessed on hens from the Y11 resistant line and the L2 susceptible 
      line, orally inoculated with 10(8) CFU S. Enteritidis per animal. 
      Anti-lipopolysaccharide (LPS) IgG titres were higher but the liver and spleen 
      were less contaminated in hens from the Y11 line than in hens from the L2 line (p 
      = 0.013, 0.031 and 0.026 respectively). In the second experiment, the hens were 
      inoculated orally with 1.7 x 10(8) CFU S. Enteritidis per animal in order to 
      select the ELISA methods showing the more significant differences. ELISAs were 
      based on LPS, flagella, LPS from rough (LPS-R) and smooth strains (LPS-S) and 
      detected IgG and IgM antibodies from sera and yolks. No between-line host 
      response variation was observed in the yolk, with LPS-S and R antigens nor with 
      anti-LPS IgM in the sera. Otherwise, significant differences were encountered 
      between hen lines with the ELISAs performed on the sera detecting anti-LPS IgG, 
      anti-flagella IgG or IgM (p = 0.017, 0.017 and p < 0.001 respectively). When 
      comparing the kinetics of the selected ELISAs, the IgG antibodies against LPS 
      detected between-line variations as early as 1 to 4 weeks pi, whereas with IgG 
      against flagella, the differences were only detected at 1 and 2 weeks pi and with 
      IgM against flagella, the differences were significant at 1, 2, 4 and 8 weeks pi. 
      In conclusion, resistant hen lines producing higher levels of antibodies than the 
      susceptible hen lines may be selected with these ELISAs.
FAU - Proux, Karine
AU  - Proux K
AD  - AFSSA Ploufragan, BP, France. k.proux@ploufragan.afssa.fr
FAU - Jouy, Eric
AU  - Jouy E
FAU - Houdayer, Catherine
AU  - Houdayer C
FAU - Protais, Jocelyne
AU  - Protais J
FAU - Dibb-Fuller, Mike
AU  - Dibb-Fuller M
FAU - Boscher, Evelyne
AU  - Boscher E
FAU - Gillard, Angelique
AU  - Gillard A
FAU - Gracieux, Patrice
AU  - Gracieux P
FAU - Gilbert, Florence
AU  - Gilbert F
FAU - Beaumont, Catherine
AU  - Beaumont C
FAU - Duchet-Suchaux, Marion
AU  - Duchet-Suchaux M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antibodies, Bacterial/analysis/biosynthesis
MH  - *Chickens
MH  - Colony Count, Microbial
MH  - Disease Susceptibility
MH  - Enzyme-Linked Immunosorbent Assay/methods/*veterinary
MH  - Female
MH  - Flagella/immunology
MH  - Immunoglobulin G/analysis/biosynthesis
MH  - Immunoglobulin M/analysis/biosynthesis
MH  - Lipopolysaccharides/immunology
MH  - Male
MH  - Poultry Diseases/diagnosis/*immunology
MH  - Reproducibility of Results
MH  - Salmonella Infections, Animal/diagnosis/*immunology
MH  - Salmonella enteritidis/*immunology
MH  - Sensitivity and Specificity
MH  - Specific Pathogen-Free Organisms
EDAT- 2002/03/05 10:00
MHDA- 2002/06/19 10:01
CRDT- 2002/03/05 10:00
PHST- 2002/03/05 10:00 [pubmed]
PHST- 2002/06/19 10:01 [medline]
PHST- 2002/03/05 10:00 [entrez]
AID - 10.1051/vetres:2001003 [doi]
PST - ppublish
SO  - Vet Res. 2002 Jan-Feb;33(1):23-33. doi: 10.1051/vetres:2001003.