PMID- 11874561
OWN - NLM
STAT- MEDLINE
DCOM- 20020612
LR  - 20190916
IS  - 0141-9838 (Print)
IS  - 0141-9838 (Linking)
VI  - 24
IP  - 2
DP  - 2002 Feb
TI  - Chemokine and cytokine expression in murine intestinal epithelium following 
      Nippostrongylus brasiliensis infection.
PG  - 67-75
AB  - Infection of mice with the nematode parasite Nippostrongylus brasiliensis results 
      in a well characterized intestinal mastocytosis with intraepithelial migration of 
      mucosal mast cells (MMC). The molecules mediating this response are unknown. We 
      examined expression of several putative mast cell chemoattractants in intestinal 
      epithelium following N. brasiliensis infection. Expression of the chemokines 
      monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1 
      alpha (MIP-1alpha), RANTES (regulated on activation normal T-cell expressed and 
      secreted), fractalkine, and thymocyte expressed chemokine (TECK); and the 
      cytokines stem cell factor (SCF) and transforming growth factor beta1 (TGFbeta1), 
      was constitutive and no alteration was detected following infection. MCP-1 
      expression was also constitutive but at much lower levels and increased 
      expression was detected on days 7 and 14 postinfection. Expression of MCP-1 in 
      whole jejunum was at much higher levels than in epithelium. Constitutive 
      expression of MCP-1, MIP-1alpha and TGFbeta1 was also detected in cultured bone 
      marrow-derived homologues of MMC. In an intestinal epithelial cell line (CMT-93), 
      there was constitutive expression of SCF, TGFalpha1, fractalkine and MCP-1. The 
      results show that, in vivo, epithelium is a potentially important source of mast 
      cell chemoattractants.
FAU - Rosbottom, Anne
AU  - Rosbottom A
AD  - Department of Veterinary Pathology, University of Edinburgh, Easter Bush 
      Veterinary Centre, Roslin, Midlothian, UK.
FAU - Knight, Pamela A
AU  - Knight PA
FAU - McLachlan, Gerry
AU  - McLachlan G
FAU - Thornton, Elizabeth M
AU  - Thornton EM
FAU - Wright, Steven W
AU  - Wright SW
FAU - Miller, Hugh R P
AU  - Miller HR
FAU - Scudamore, Cheryl L
AU  - Scudamore CL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Parasite Immunol
JT  - Parasite immunology
JID - 7910948
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokines/*metabolism
MH  - Cytokines/*metabolism
MH  - Intestinal Mucosa/*immunology/parasitology/ultrastructure
MH  - Jejunum/immunology/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nippostrongylus/*physiology
MH  - Parasite Egg Count
MH  - Sequence Homology
MH  - Strongylida Infections/*immunology/parasitology/pathology
EDAT- 2002/03/05 10:00
MHDA- 2002/06/13 10:01
CRDT- 2002/03/05 10:00
PHST- 2002/03/05 10:00 [pubmed]
PHST- 2002/06/13 10:01 [medline]
PHST- 2002/03/05 10:00 [entrez]
AID - 437 [pii]
AID - 10.1046/j.0141-9838.2001.00437.x [doi]
PST - ppublish
SO  - Parasite Immunol. 2002 Feb;24(2):67-75. doi: 10.1046/j.0141-9838.2001.00437.x.