PMID- 11876976
OWN - NLM
STAT- MEDLINE
DCOM- 20031030
LR  - 20061115
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 21
IP  - 4
DP  - 2000 Apr
TI  - [Detection of trisomy 8 with interphase fluorescence in situ hybridization in 
      myelodysplastic syndromes].
PG  - 179-81
AB  - OBJECTIVE: To explore the value of interphase fluorescence in situ hybridization 
      (FISH) in the detection of trisomy 8 in myelodysplastic syndromes (MDS). METHODS: 
      Conventional cytogenetics (CC) and interphase FISH using SpectrumGreen labelled 
      chromosome 8 centromere specific probe were simultaneously carried out to detect 
      trisomy 8 in 69 MDS and 6 normal individuals. RESULTS: Two hundred interphase 
      cells were counted and cells with three green hybridization spots > 3% was 
      assigned. Eleven cases displayed trisomy 8 by CC and were confirmed in 10 by 
      FISH. In 7 cases, the percentage of trisomy 8 cells was significantly lower by 
      FISH than by CC. Seven cases displayed trisomy 8 by FISH in 58 cases who did not 
      show trisomy 8 by CC. Of the 7 cases, two had 2 and 3 marker chromosomes 
      respectively, 4 had normal karyotypes. CONCLUSIONS: Interphase FISH was a useful 
      method for the detection of trisomy 8 in MDS, especially in patients with normal 
      karyotype or marker chromosome. It was a important complement to CC.
FAU - Li, J
AU  - Li J
AD  - First Affiliated Hospital of Suzhou Medical College, Jiangsu Institute of 
      Hematology, Suzhou 215006, China.
FAU - Pan, J
AU  - Pan J
FAU - Xue, Y
AU  - Xue Y
FAU - Wu, C
AU  - Wu C
FAU - Xie, X
AU  - Xie X
FAU - Guo, Y
AU  - Guo Y
FAU - Ruan, C
AU  - Ruan C
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosomes, Human, Pair 8
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
MH  - *Trisomy
EDAT- 2002/03/06 10:00
MHDA- 2003/10/31 05:00
CRDT- 2002/03/06 10:00
PHST- 2002/03/06 10:00 [pubmed]
PHST- 2003/10/31 05:00 [medline]
PHST- 2002/03/06 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2000 Apr;21(4):179-81.