PMID- 11878622
OWN - NLM
STAT- MEDLINE
DCOM- 20020820
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 85
IP  - 1
DP  - 2002 Jan-Feb
TI  - Residues of oxytetracycline and its 4'-epimer in edible tissues from turkeys.
PG  - 8-14
AB  - Residues of oxytetracycline (OTC) in edible tissues (muscle, liver, and kidney) 
      of 18 turkeys were determined after continuous administration of the drug for 3 
      days in drinking water at the maximum recommended concentration of 400 mg/L. The 
      European Union (EU) maximum residue limits (MRLs) set for OTC are 100 microg/kg 
      in muscle tissues, 300 microg/kg in liver, and 600 microg/kg in kidney, as the 
      sum of the parent compound and its derivative 4'-epi-oxytetracycline (4-epi-OTC). 
      Cleanup of tissue samples was performed by metal chelate affinity chromatography 
      (MCAC), but the original technique was miniaturized by the adoption of a mini 
      solid-phase extraction column, allowing reduction of solvents, time, and 
      hazardous waste. OTC and its 4'-epimer were quantitated by an isocratic liquid 
      chromatography elution with UV detection. After 1 day of withdrawal, OTC plus 
      4-epi-OTC residues were greater than MRL values in muscle and liver; 3 days after 
      the end of treatment, all tissue residues were far lower than the MRL values. At 
      the first day after the end of treatment, 4-epi-OTC was detected at very low 
      concentrations only in muscle, in liver after 1 and 3 days of withdrawal, and in 
      kidney at all sampling times. The withdrawal time was calculated according to EU 
      recommendations and was set at 5 days.
FAU - Capolongo, Francesca
AU  - Capolongo F
AD  - Universita degli Studi di Padova, Istituto di Patologia e Igiene Veterinaria, 
      Agripolis Legnaro Padova, Italy.
FAU - Santi, Annalisa
AU  - Santi A
FAU - Tomasi, Luciana
AU  - Tomasi L
FAU - Anfossi, Paola
AU  - Anfossi P
FAU - Missagia, Mauro
AU  - Missagia M
FAU - Montesissa, Clara
AU  - Montesissa C
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Solutions)
RN  - X20I9EN955 (Oxytetracycline)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, Liquid
MH  - Drug Residues
MH  - Female
MH  - Indicators and Reagents
MH  - Isomerism
MH  - Kidney/chemistry
MH  - Liver/chemistry
MH  - Male
MH  - Meat/*analysis
MH  - Muscle, Skeletal/chemistry
MH  - Oxytetracycline/*analysis
MH  - Reproducibility of Results
MH  - Solutions
MH  - Turkeys/*metabolism
EDAT- 2002/03/07 10:00
MHDA- 2002/08/21 10:01
CRDT- 2002/03/07 10:00
PHST- 2002/03/07 10:00 [pubmed]
PHST- 2002/08/21 10:01 [medline]
PHST- 2002/03/07 10:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 2002 Jan-Feb;85(1):8-14.