PMID- 11880178
OWN - NLM
STAT- MEDLINE
DCOM- 20020515
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 179
IP  - 1
DP  - 2002 May 8
TI  - Cellular thiol status-dependent inhibition of tumor cell growth via modulation of 
      retinoblastoma protein phosphorylation by (-)-epigallocatechin.
PG  - 25-32
AB  - Tea polyphenols have been shown to inhibit tumor cell growth, but there is 
      limited information on their effects on cell signaling and cell cycle control 
      pathways. We have shown the involvement of such mechanisms as activation of 
      mitogenic activated protein kinases, decreases in ornithine decarboxylase 
      activity and in cellular thiol levels, elicitation of mitochondrial cytochrome c 
      release, and activation of caspases by the green tea galloyl polyphenol, 
      epigallocatechin (EGC). In the current study, we sought to determine how EGC 
      alters cell cycle and its related control factors in its growth inhibitory effect 
      in Ehrlich ascites tumor cells. The significant finding here is that EGC caused a 
      dose-dependent accumulation of cells in the G1 phase and a decrease in the 
      phosphorylation of the retinoblastoma (Rb) protein, which was also in a cellular 
      thiol-dependent manner. The involvement of a cellular thiol-dependent modulation 
      in Rb phosphorylation leading to the regulation of tumor cell growth by a green 
      tea polyphenol is a novel observation, to the best of our knowledge.
FAU - Kennedy, David Opare
AU  - Kennedy DO
AD  - Department of Food and Human Health Sciences, Graduate School of Human Life 
      Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, 
      Japan.
FAU - Kojima, Akiko
AU  - Kojima A
FAU - Moffatt, Jerry
AU  - Moffatt J
FAU - Yamagiwa, Hitoshi
AU  - Yamagiwa H
FAU - Yano, Yoshihisa
AU  - Yano Y
FAU - Hasuma, Tadayoshi
AU  - Hasuma T
FAU - Otani, Shuzo
AU  - Otani S
FAU - Matsui-Yuasa, Isao
AU  - Matsui-Yuasa I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Plant Extracts)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Sulfhydryl Compounds)
RN  - 0 (Tea)
RN  - 8R1V1STN48 (Catechin)
RN  - HEJ6575V1X (gallocatechol)
SB  - IM
MH  - Animals
MH  - Carcinoma, Ehrlich Tumor/*drug therapy/metabolism/pathology
MH  - Catechin/*analogs & derivatives/*pharmacology
MH  - Cell Cycle/*drug effects
MH  - Cell Division/*drug effects
MH  - Cell Survival/drug effects
MH  - Phosphorylation/*drug effects
MH  - Plant Extracts/pharmacology
MH  - Retinoblastoma Protein/*metabolism
MH  - Sulfhydryl Compounds/*metabolism
MH  - Tea
MH  - Tumor Cells, Cultured
EDAT- 2002/03/07 10:00
MHDA- 2002/05/16 10:01
CRDT- 2002/03/07 10:00
PHST- 2002/03/07 10:00 [pubmed]
PHST- 2002/05/16 10:01 [medline]
PHST- 2002/03/07 10:00 [entrez]
AID - S0304383501008564 [pii]
AID - 10.1016/s0304-3835(01)00856-4 [doi]
PST - ppublish
SO  - Cancer Lett. 2002 May 8;179(1):25-32. doi: 10.1016/s0304-3835(01)00856-4.