PMID- 11881731
OWN - NLM
STAT- MEDLINE
DCOM- 20020425
LR  - 20190916
IS  - 1320-5463 (Print)
IS  - 1320-5463 (Linking)
VI  - 51
IP  - 10
DP  - 2001 Oct
TI  - Detection of numerical alterations of chromosome 1 in cytopathological specimens 
      of breast tumors by chromogen in situ hybridization.
PG  - 786-91
AB  - To investigate the effectiveness of chromogen in situ hybridization (CISH) in the 
      diagnosis of breast tumors, numerical alterations of chromosome 1 were examined 
      by CISH and fluorescence in situ hybridization (FISH) methods, and the presence 
      of der(16)t(1;16) was also examined by FISH in imprinted cytology specimens from 
      resected tissues of 14 carcinomas and five non-malignant lesions. The modal 
      signal counts of chromosome 1 were compared between the specimens processed by 
      CISH and FISH for each case. Aneusomies of the long arm of chromosome 1 were 
      detected in 10 (71%) carcinomas as the major clones by both methods. In addition, 
      one atypical papilloma demonstrated tetrasomy of 1q12 as a major clone by CISH, 
      but such a clone was at first overlooked by FISH. Four other benign lesions 
      showed disomic 1q12 signals as a major clone by both CISH and FISH. As additional 
      information from FISH, eight cancers showed structural or numerical alterations 
      of chromosome 16, and four showed der(16)t(1;16). In total, 10 carcinomas showed 
      chromosome 16 alterations, and all of these overlapped with the carcinomas with 
      1q12 aneusomies. The CISH method provided almost the same results as the FISH 
      method, and both methods were considered applicable in supportive diagnosis of 
      cytological specimens of breast tumors. In addition, the CISH method was superior 
      in the detection of numerical alterations in carcinoma cells by referring to the 
      morphology of cells and in the detection of significant clones which might be 
      missed under dark-field microscopy.
FAU - Takarabe, T
AU  - Takarabe T
AD  - Pathology Division, National Cancer Center Research Institute and Hospital, 
      Tokyo, Japan.
FAU - Tsuda, H
AU  - Tsuda H
FAU - Okada, S
AU  - Okada S
FAU - Fukutomi, T
AU  - Fukutomi T
FAU - Hirohashi, S
AU  - Hirohashi S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - Carcinoma in Situ/*genetics/pathology
MH  - Carcinoma, Intraductal, Noninfiltrating/*genetics/pathology
MH  - Cell Nucleus/genetics/pathology
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 1
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Reproducibility of Results
EDAT- 2002/03/08 10:00
MHDA- 2002/04/26 10:01
CRDT- 2002/03/08 10:00
PHST- 2002/03/08 10:00 [pubmed]
PHST- 2002/04/26 10:01 [medline]
PHST- 2002/03/08 10:00 [entrez]
AID - 10.1046/j.1440-1827.2001.01282.x [doi]
PST - ppublish
SO  - Pathol Int. 2001 Oct;51(10):786-91. doi: 10.1046/j.1440-1827.2001.01282.x.