PMID- 11882518
OWN - NLM
STAT- MEDLINE
DCOM- 20020408
LR  - 20131121
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 282
IP  - 4
DP  - 2002 Apr
TI  - Shedding of TNF-alpha receptors, blood pressure, and insulin sensitivity in type 
      2 diabetes mellitus.
PG  - E952-9
AB  - Tumor necrosis factor-alpha (TNF-alpha) is increasingly recognized as a key 
      component in the development of insulin resistance and increased blood pressure. 
      In a sample of 368 individuals, the ratio of soluble TNF-alpha receptors 
      (sTNFR2/sTNFR1) correlated positively with systolic and diastolic blood pressure 
      (P < 0.01). This ratio was significantly greater in type 2 diabetic subjects 
      (DM-2) than in type 1 diabetic patients and was greater than in control 
      nondiabetic subjects (P < 0.00001). The TNF-alpha receptor 1 (TNFR1) density in 
      peripheral blood monocytes was similar in DM-2 patients and in nondiabetic 
      subjects. After phorbol 12-myristate 13-acetate, TNFR1 shedding was significantly 
      decreased in DM-2 compared with control subjects, and it was directly associated 
      with insulin sensitivity (r = 0.54, P = 0.03). Serum sTNFR1 concentration was 
      also linked to the vasodilatory response to glyceryltrinitrate (P = 0.01). 
      Conversely, TNF-alpha receptor 2 shedding was negatively associated with insulin 
      sensitivity (r = -0.54, P = 0.03), whereas shedding of L-selectin showed no 
      significant association. After exercise-induced lowering of blood pressure, a 
      parallel decrease in sTNFR2/sTNFR1 was observed in DM-2 patients. Our findings 
      suggest that insulin resistance and blood pressure are linked to altered shedding 
      of TNF-alpha receptors in DM-2. The latter seems reversible and is not 
      genetically determined.
FAU - Fernandez-Real, Jose-Manuel
AU  - Fernandez-Real JM
AD  - Unitat de Diabetologia, Endocrinologia i Nutricio, University Hospital of Girona, 
      17001 Girona, Spain. endocrino@htrueta.scs.es
FAU - Lainez, Begona
AU  - Lainez B
FAU - Vendrell, Joan
AU  - Vendrell J
FAU - Rigla, Mercedes
AU  - Rigla M
FAU - Castro, Antoni
AU  - Castro A
FAU - Penarroja, Georgina
AU  - Penarroja G
FAU - Broch, Montserrat
AU  - Broch M
FAU - Perez, Antonio
AU  - Perez A
FAU - Richart, Cristobal
AU  - Richart C
FAU - Engel, Pablo
AU  - Engel P
FAU - Ricart, Wifredo
AU  - Ricart W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Antigens, CD)
RN  - 0 (Insulin)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type II)
RN  - G59M7S0WS3 (Nitroglycerin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/*blood
MH  - *Blood Pressure
MH  - Body Mass Index
MH  - Diabetes Mellitus, Type 1/physiopathology
MH  - Diabetes Mellitus, Type 2/*physiopathology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Insulin/*pharmacology
MH  - Insulin Resistance
MH  - Male
MH  - Middle Aged
MH  - Monocytes/chemistry
MH  - Nitroglycerin/pharmacology
MH  - Receptors, Tumor Necrosis Factor/*blood
MH  - Receptors, Tumor Necrosis Factor, Type I
MH  - Receptors, Tumor Necrosis Factor, Type II
MH  - Solubility
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Vasodilation/drug effects
EDAT- 2002/03/08 10:00
MHDA- 2002/04/09 10:01
CRDT- 2002/03/08 10:00
PHST- 2002/03/08 10:00 [pubmed]
PHST- 2002/04/09 10:01 [medline]
PHST- 2002/03/08 10:00 [entrez]
AID - 10.1152/ajpendo.00444.2001 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2002 Apr;282(4):E952-9. doi: 
      10.1152/ajpendo.00444.2001.