PMID- 11882907
OWN - NLM
STAT- MEDLINE
DCOM- 20020523
LR  - 20191105
IS  - 1219-4956 (Print)
IS  - 1219-4956 (Linking)
VI  - 7
IP  - 4
DP  - 2001
TI  - Her-2/neu gene amplification compared with HER-2/neu protein overexpression on 
      ultrasound guided core-needle biopsy specimens of breast carcinoma.
PG  - 279-83
AB  - Genomic amplification and oncoprotein overexpression of Her-2/neu was studied on 
      ultrasound core needle biopsy specimens of the infiltrative ductal carcinomas of 
      the breast. We performed two colour fluorescence in situ hybridization (FISH) for 
      Her-2/neu and chromosome 17 and compared the FISH results with the 
      immunohistochemical overexpression of Her-2/neu protein by 2 antibodies (DAKO 
      HercepTest and the BioGenex monoclonal antibody AM 134-5M). Furthermore, 
      following radical mastectomy with axillary dissection, Her-2/neu status of the 
      patients were compared with the well known histopathological prognostic factors 
      such as histologic grade, tumor stage, lympho/ vascular invasion, surgical margin 
      status and Paget s disease. Amplification was demonstrated 27% of the cases. 
      Her-2/neu protein overexpression was detected in 47% and 80% of the cases with 
      CB11 and HercepTest respectively. We revealed statistically significant 
      association between the tumor, oncoprotein expression and oncogene amplification 
      (p<0.05). The results of our study showed that combination of IHC and FISH 
      methods enhances the evaluation of tumor genetics at both gene and protein level 
      for the analysis of Her-2/neu in breast carcinoma.
FAU - Kaya, H
AU  - Kaya H
AD  - Marmara University Hospital, Department of Pathology Altunizade, Istanbul, 81190, 
      Turkey. hkaya@superonline.com
FAU - Ragazzini, T
AU  - Ragazzini T
FAU - Aribal, E
AU  - Aribal E
FAU - Guney, I
AU  - Guney I
FAU - Kotiloglu, E
AU  - Kotiloglu E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Switzerland
TA  - Pathol Oncol Res
JT  - Pathology oncology research : POR
JID - 9706087
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biopsy, Needle
MH  - Breast Neoplasms/*genetics/*metabolism/pathology
MH  - Carcinoma, Ductal, Breast/*genetics/*metabolism/pathology
MH  - Female
MH  - Gene Amplification
MH  - Gene Expression
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Paget's Disease, Mammary/metabolism/pathology
MH  - Receptor, ErbB-2/*genetics/*metabolism
MH  - Ultrasonography, Mammary
EDAT- 2002/03/08 10:00
MHDA- 2002/05/25 10:01
CRDT- 2002/03/08 10:00
PHST- 2002/03/08 10:00 [pubmed]
PHST- 2002/05/25 10:01 [medline]
PHST- 2002/03/08 10:00 [entrez]
AID - PAOR.2001.7.4.0279 [pii]
AID - 10.1007/BF03032384 [doi]
PST - ppublish
SO  - Pathol Oncol Res. 2001;7(4):279-83. doi: 10.1007/BF03032384.