PMID- 11884223
OWN - NLM
STAT- MEDLINE
DCOM- 20031125
LR  - 20171116
IS  - 1043-6618 (Print)
IS  - 1043-6618 (Linking)
VI  - 45
IP  - 3
DP  - 2002 Mar
TI  - A study on the relationship between homocysteine and diabetic nephropathy in 
      rats.
PG  - 249-52
AB  - Hyperhomocysteinemia is known to be associated with many of the occlusive 
      vascular diseases including ischemic heart disease. Elevated plasma total 
      homocysteine (t-Hcy) is also remarkably common among patients with moderate to 
      severe renal failure. The purpose of this study was to investigate the role of 
      homocysteine (Hcy) in the pathogenesis of diabetic nephropathy in the rat. 
      Additionally, any effect of aminoguanidine (AG), an inhibitor of advanced 
      glycation end product (AGE) formation, on the onset of nephropathic symptoms and 
      on the concentrations of Hcy was searched for. Diabetes was induced in male 
      Wistar albino rats (6 months old) by a single injection of 50 mg x kg 
      (-1)streptozotocin (STZ) into the penile vein. Animals with blood glucose levels 
      higher than 350 mg x dl (-1)72 h after STZ injection were included in the study. 
      Age-matched rats receiving a single dose of citrate buffer served as controls. 
      One half of the control and diabetic groups received AG via drinking water (1 g l 
      (-1)). The experimental period lasted for ten weeks. Animals were killed by 
      cardiac venipuncture after 24 hour urine samples were collected. Serum t-Hcy was 
      quantified using HPLC, and urinary GAGs using the spectrophotometric 1,9-dimethyl 
      methylene blue dye method. Serum glucose, protein, creatinine and total sulfydryl 
      (t-SH) measurements, and urinary protein determinations were carried out 
      spectrophotometrically. In diabetic rats, serum t-Hcy levels were significantly 
      decreased (P< 0.001), and were negatively correlated with the urinary protein 
      concentration (r= -0.67, P< 0.05). Urinary GAG levels were also increased in 
      diabetic rats (P< 0.001). AG neither affected the t-Hcy levels, nor ameliorated 
      the nephropathic symptoms. These results indicate that diabetic nephropathy is 
      not linked to homocysteinemia in the rat.
CI  - Copyright 2002 Elsevier Science Ltd.
FAU - Unlucerci, Yesim
AU  - Unlucerci Y
AD  - Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, 
      Istanbul, Capa 34390, Turkey. yesimunlucerci@hotmail.com
FAU - Bekpinar, Selda
AU  - Bekpinar S
FAU - Gurdol, Figen
AU  - Gurdol F
FAU - Seferoglu, Gulay
AU  - Seferoglu G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Pharmacol Res
JT  - Pharmacological research
JID - 8907422
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Guanidines)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - AYI8EX34EU (Creatinine)
RN  - SCQ4EZQ113 (pimagedine)
SB  - IM
MH  - Animals
MH  - Creatinine/blood/urine
MH  - Diabetic Nephropathies/*blood/urine
MH  - Enzyme Inhibitors/pharmacology
MH  - Glycation End Products, Advanced/blood/urine
MH  - Glycosaminoglycans/urine
MH  - Guanidines/pharmacology
MH  - Homocysteine/*blood
MH  - Male
MH  - Rats
MH  - Rats, Wistar
EDAT- 2002/03/09 10:00
MHDA- 2003/12/03 05:00
CRDT- 2002/03/09 10:00
PHST- 2002/03/09 10:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2002/03/09 10:00 [entrez]
AID - S1043661801909429 [pii]
AID - 10.1006/phrs.2001.0942 [doi]
PST - ppublish
SO  - Pharmacol Res. 2002 Mar;45(3):249-52. doi: 10.1006/phrs.2001.0942.