PMID- 11884547
OWN - NLM
STAT- MEDLINE
DCOM- 20020419
LR  - 20220330
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 76
IP  - 7
DP  - 2002 Apr
TI  - Experimental inoculation of conventional pigs with porcine reproductive and 
      respiratory syndrome virus and porcine circovirus 2.
PG  - 3232-9
AB  - Postweaning multisystemic wasting syndrome (PMWS) is a disease of nursery and 
      fattening pigs characterized by growth retardation, paleness of the skin, 
      dyspnea, and increased mortality rates. Porcine circovirus 2 (PCV2) has been 
      demonstrated to be the cause of PMWS. However, other factors are needed for full 
      development of the syndrome, and porcine reproductive and respiratory syndrome 
      virus (PRRSV) infection has been suggested to be one of them. Twenty-four 
      conventional 5-week-old pigs were distributed in four groups: control (n = 5), 
      PRRSV inoculated (n = 5), PCV2 inoculated (n = 7), and PRRSV and PCV2 inoculated 
      (n = 7). The two groups inoculated with PRRSV showed growth retardation. Pigs 
      inoculated with both PRRSV and PCV2 had increased rectal temperature. One of 
      these pigs developed wasting, had severe respiratory distress, and died. The most 
      important microscopic lesion in pigs inoculated with PCV2 was lymphocyte 
      depletion with histiocytic infiltration of the lymphoid organs, more severe and 
      in a wider range of tissues in doubly inoculated pigs. Interstitial pneumonia was 
      observed in the three inoculated groups. PCV2 nucleic acid was found by in situ 
      hybridization in larger amounts and in a wider range of lymphoid tissues in 
      PRRSV- and PCV2-inoculated than in PCV2-inoculated pigs. TaqMan PCR was performed 
      to quantify the PCV2 loads in serum during the experiment. PCV2 loads were higher 
      in doubly inoculated pigs than in pigs inoculated with PCV2 alone. These findings 
      indicate that severe disease can be reproduced in conventional 5-week-old pigs by 
      inoculation of PRRSV and PCV2. Moreover, these results support the hypothesis 
      that PRRSV infection enhances PCV2 replication.
FAU - Rovira, A
AU  - Rovira A
AD  - Centre de Recerca en Sanitat Animal (CReSA), Departament de Sanitat i d'Anatomia 
      Animals, Universitat Autonoma de Barcelona, Barcelona, Spain. 
      alberto.rovira@campus.uab.es
FAU - Balasch, M
AU  - Balasch M
FAU - Segales, J
AU  - Segales J
FAU - Garcia, L
AU  - Garcia L
FAU - Plana-Duran, J
AU  - Plana-Duran J
FAU - Rosell, C
AU  - Rosell C
FAU - Ellerbrok, H
AU  - Ellerbrok H
FAU - Mankertz, A
AU  - Mankertz A
FAU - Domingo, M
AU  - Domingo M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
RN  - EC 2.7.7.- (Taq Polymerase)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/isolation & purification
MH  - Circoviridae Infections/*veterinary
MH  - *Circovirus
MH  - DNA, Viral/isolation & purification
MH  - Fever/etiology
MH  - Lung Diseases, Interstitial/etiology/pathology
MH  - Lymphoid Tissue/pathology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*pathology/virology
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - Swine Diseases/blood/*pathology/virology
MH  - Taq Polymerase
MH  - Viral Load
MH  - Viremia
MH  - Wasting Syndrome/*veterinary
MH  - Weaning
MH  - Weight Loss
PMC - PMC136035
EDAT- 2002/03/09 10:00
MHDA- 2002/04/20 10:01
PMCR- 2002/04/01
CRDT- 2002/03/09 10:00
PHST- 2002/03/09 10:00 [pubmed]
PHST- 2002/04/20 10:01 [medline]
PHST- 2002/03/09 10:00 [entrez]
PHST- 2002/04/01 00:00 [pmc-release]
AID - 1885 [pii]
AID - 10.1128/jvi.76.7.3232-3239.2002 [doi]
PST - ppublish
SO  - J Virol. 2002 Apr;76(7):3232-9. doi: 10.1128/jvi.76.7.3232-3239.2002.