PMID- 11886555
OWN - NLM
STAT- MEDLINE
DCOM- 20020507
LR  - 20190725
IS  - 0950-382X (Print)
IS  - 0950-382X (Linking)
VI  - 42
IP  - 5
DP  - 2001 Dec
TI  - Sister chromosome cohesion of Escherichia coli.
PG  - 1233-41
AB  - We analysed Escherichia coli cells synchronized for initiation of chromosomal DNA 
      replication by fluorescence in situ hybridization (FISH) using fluorescent DNA 
      probes corresponding to various chromosomal regions. Sister copies of regions in 
      an approximately oriC-proximal half of the chromosome are cohesive with each 
      other after replication until the late period of chromosome replication. Sister 
      copies of regions relatively close to the terminus are also separated from each 
      other in the same late period of replication. It is important that sister copies 
      in all the tested regions are thus separated from each other nearly all at once 
      in the late period of chromosome replication. These results are consistent with 
      results obtained by FISH in randomly growing cultures. Cohesion of sister copies 
      in an oriC-close region is observed in a dam null mutant lacking DNA adenine 
      methyltransferase the same as in the parental isogenic dam+ strain, indicating 
      that the cohesion is independent of DNA adenine methyltransferase. This further 
      implies that hemimethylated DNA-binding proteins, such as SeqA, are not involved 
      in the cohesion. On the other hand, the cohesion of sister copies of the 
      oriC-close region was not observed in mukB null mutant cells, suggesting that 
      MukB might be involved in the chromosome cohesion.
FAU - Sunako, Y
AU  - Sunako Y
AD  - Department of Molecular Cell Biology, Institute of Molecular Embryology and 
      Genetics, Kumamoto University, Kuhonji 4-24-1, Kumamoto 862-0976, Japan.
FAU - Onogi, T
AU  - Onogi T
FAU - Hiraga, S
AU  - Hiraga S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Microbiol
JT  - Molecular microbiology
JID - 8712028
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes, Bacterial/*genetics
MH  - DNA Replication/*genetics
MH  - Escherichia coli/*genetics
MH  - In Situ Hybridization, Fluorescence
EDAT- 2002/03/12 10:00
MHDA- 2002/05/08 10:01
CRDT- 2002/03/12 10:00
PHST- 2002/03/12 10:00 [pubmed]
PHST- 2002/05/08 10:01 [medline]
PHST- 2002/03/12 10:00 [entrez]
AID - 2680 [pii]
AID - 10.1046/j.1365-2958.2001.02680.x [doi]
PST - ppublish
SO  - Mol Microbiol. 2001 Dec;42(5):1233-41. doi: 10.1046/j.1365-2958.2001.02680.x.