PMID- 11886884
OWN - NLM
STAT- MEDLINE
DCOM- 20020523
LR  - 20201208
IS  - 0022-0957 (Print)
IS  - 0022-0957 (Linking)
VI  - 53
IP  - 369
DP  - 2002 Apr
TI  - Identification of programmed cell death in situ in individual plant cells in vivo 
      using a chromosome preparation technique.
PG  - 651-8
AB  - A simple procedure, which combines a chromosome preparation technique with an in 
      situ labelling technique modified from fluorescence in situ hybridization (FISH), 
      has been developed for in situ detection of plant programmed cell death (PCD) at 
      the single-cell level. After exposure of chromosomes and nuclei on slides by 
      enzymolysis, Klenow or TdT was used to incorporate Bio-dUTP or fluorescein-dUTP 
      at sites of DNA breaks. After Klenow-mediated labelling, the signals were 
      amplified by a cascade of antigen-antibody reaction according to the detection 
      system of FISH. This method enables in situ detection of plant PCD in vivo 
      morphologically and biochemically at the chromosome, nuclear and DNA levels 
      without cell culture and histological sectioning. This technique permits 
      labelling of DNA breaks with high sensitivity due to increased chromosome and 
      nucleus exposure to the labelling solutions, as well as due to the immunological 
      amplification of the signals. Moreover, the changes in the cells were easier to 
      be observed because the spatial obstacle of the cell wall and its 
      autofluorescence were eliminated. It is potentially useful for in situ detection 
      of PCD in plant root meristematic cells triggered by various environmental 
      abiotic factors. It is proposed that the root tip is a versatile in vivo system 
      for studying PCD induced by environmental abiotic factors.
FAU - Ning, Shun-Bin
AU  - Ning SB
AD  - Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan, 
      430072, People's Republic of China.
FAU - Wang, Ling
AU  - Wang L
FAU - Song, Yun-Chun
AU  - Song YC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
RN  - EC 2.7.7.7 (DNA Polymerase I)
SB  - IM
MH  - Apoptosis/*genetics
MH  - Cell Nucleus/genetics/metabolism
MH  - Chromosomes/*genetics
MH  - DNA Polymerase I/metabolism
MH  - In Situ Hybridization, Fluorescence/methods
MH  - In Situ Nick-End Labeling/*methods
MH  - Karyotyping
MH  - Meristem/genetics/physiology
MH  - Plant Roots/genetics/*physiology
MH  - Signal Transduction
MH  - Zea mays/genetics/*physiology
EDAT- 2002/03/12 10:00
MHDA- 2002/05/25 10:01
CRDT- 2002/03/12 10:00
PHST- 2002/03/12 10:00 [pubmed]
PHST- 2002/05/25 10:01 [medline]
PHST- 2002/03/12 10:00 [entrez]
AID - 10.1093/jexbot/53.369.651 [doi]
PST - ppublish
SO  - J Exp Bot. 2002 Apr;53(369):651-8. doi: 10.1093/jexbot/53.369.651.