PMID- 11888521
OWN - NLM
STAT- MEDLINE
DCOM- 20020514
LR  - 20190718
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 161
IP  - 2
DP  - 2002 Apr
TI  - Inhibition of TNF-alpha induced ICAM-1, VCAM-1 and E-selectin expression by 
      selenium.
PG  - 381-6
AB  - The initiation of an atherosclerotic lesion involves an endothelial cell 
      pro-inflammatory state that recruits leukocytes and promotes their movement 
      across the endothelium. These processes require endothelial expression of 
      intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 
      (VCAM-1), and endothelial-leukocyte adhesion molecule-1 (E-selectin). Tumor 
      necrosis factor-alpha (TNF-alpha) is a powerful inducer of these adhesion 
      molecules. Selenium status is known to affect the rate of atherosclerosis. These 
      experiments tested whether selenium alters cytokine-induced expression of these 
      adhesion molecules. Human umbilical vein endothelial cells (HUVECs) were 
      pretreated for 24 h with sodium selenite (0-2 microM) and then treated with 0 or 
      50 U/ml TNF-alpha in the presence of 0-2 microM selenite. ICAM-1, VCAM-1 and 
      E-selectin were detected by ELISA and their mRNAs were evaluated by Northern 
      blots. Selenite significantly inhibited TNF-alpha-induced expression of each 
      adhesion molecule in a dose-dependent manner and reduced the level of the 
      respective mRNAs. Nuclear factor-kappa B (NF-kappa B) is required for 
      transcription of these adhesion molecule genes. Western blot analysis revealed 
      that selenite did not inhibit the translocation of the p65 subunit of NF-kappa B 
      to the nucleus. In conclusion, these data indicate selenium can modulate 
      cytokine-induced expression of ICAM-1, VCAM-1 and E-selectin in HUVECs without 
      interfering with translocation of NF-kappa B.
FAU - Zhang, Fan
AU  - Zhang F
AD  - Department of Pharmaceutical and Biomedical Sciences, The University of Georgia, 
      Athens, GA 30602, USA.
FAU - Yu, Wei
AU  - Yu W
FAU - Hargrove, James L
AU  - Hargrove JL
FAU - Greenspan, Phillip
AU  - Greenspan P
FAU - Dean, Roger G
AU  - Dean RG
FAU - Taylor, Ethan W
AU  - Taylor EW
FAU - Hartle, Diane K
AU  - Hartle DK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (E-Selectin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Analysis of Variance
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - E-Selectin/analysis/*metabolism
MH  - Endothelium, Vascular/cytology/*drug effects/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/*analysis/drug effects
MH  - Probability
MH  - Selenium/pharmacology
MH  - Sensitivity and Specificity
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Umbilical Veins/cytology
MH  - Vascular Cell Adhesion Molecule-1/*drug effects/metabolism
EDAT- 2002/03/13 10:00
MHDA- 2002/05/15 10:01
CRDT- 2002/03/13 10:00
PHST- 2002/03/13 10:00 [pubmed]
PHST- 2002/05/15 10:01 [medline]
PHST- 2002/03/13 10:00 [entrez]
AID - S0021-9150(01)00672-4 [pii]
AID - 10.1016/s0021-9150(01)00672-4 [doi]
PST - ppublish
SO  - Atherosclerosis. 2002 Apr;161(2):381-6. doi: 10.1016/s0021-9150(01)00672-4.