PMID- 11889172
OWN - NLM
STAT- MEDLINE
DCOM- 20020408
LR  - 20171116
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 87
IP  - 3
DP  - 2002 Mar
TI  - Stimulation of autologous antitumor T-cell responses against medullary thyroid 
      carcinoma using tumor lysate-pulsed dendritic cells.
PG  - 1098-104
AB  - Dendritic cells (DCs) have attracted wide interest because of their unique 
      capacity to elicit primary and secondary antitumor responses. We have generated 
      autologous tumor lysate-pulsed DCs from three patients with medullary thyroid 
      carcinoma (MTC) and tested them for their ability to stimulate cytotoxic T-cell 
      responses against autologous MTC tumor cells in vitro. The aim of our 
      investigations was to evaluate the potential efficacy of DC-based immunotherapy 
      in patients with MTC. DCs were generated from peripheral blood monocytes using 
      GM-CSF and IL-4 (immature DCs) or GM-CSF, IL-4, and TNFalpha (mature DCs). Our 
      results indicate that mature tumor lysate-pulsed DCs are able to elicit a human 
      leukocyte antigen class I-restricted cytotoxic T-cell response against autologous 
      MTC tumor cells, whereas immature tumor lysate-pulsed DCs do not stimulate 
      significant antitumor activity. We feel that our data may be relevant for future 
      clinical trials of active immunotherapy using tumor lysate-pulsed DCs in patients 
      with MTC who have residual or distant disease after surgical treatment. The fact 
      that mature DCs displayed a substantially higher capacity to stimulate autologous 
      antitumor T-cell responses than immature DCs underlines the importance of a 
      maturation step in immunotherapy protocols based on DCs.
FAU - Bachleitner-Hofmann, T
AU  - Bachleitner-Hofmann T
AD  - Department of Surgery, University of Vienna Medical School, Vienna, Austria 1090.
FAU - Stift, A
AU  - Stift A
FAU - Friedl, J
AU  - Friedl J
FAU - Pfragner, R
AU  - Pfragner R
FAU - Radelbauer, K
AU  - Radelbauer K
FAU - Dubsky, P
AU  - Dubsky P
FAU - Schuller, G
AU  - Schuller G
FAU - Benko, T
AU  - Benko T
FAU - Niederle, B
AU  - Niederle B
FAU - Brostjan, C
AU  - Brostjan C
FAU - Jakesz, R
AU  - Jakesz R
FAU - Gnant, M
AU  - Gnant M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma/*immunology/pathology
MH  - Cell Division/physiology
MH  - Cellular Senescence/physiology
MH  - Dendritic Cells/*physiology
MH  - Female
MH  - Histocompatibility Antigens Class I/analysis
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - T-Lymphocytes, Cytotoxic/immunology/pathology/*physiology
MH  - Thyroid Neoplasms/*immunology/pathology
MH  - Tumor Cells, Cultured
EDAT- 2002/03/13 10:00
MHDA- 2002/04/09 10:01
CRDT- 2002/03/13 10:00
PHST- 2002/03/13 10:00 [pubmed]
PHST- 2002/04/09 10:01 [medline]
PHST- 2002/03/13 10:00 [entrez]
AID - 10.1210/jcem.87.3.8283 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2002 Mar;87(3):1098-104. doi: 10.1210/jcem.87.3.8283.