PMID- 11889421
OWN - NLM
STAT- MEDLINE
DCOM- 20020402
LR  - 20211203
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 73
IP  - 4
DP  - 2002 Feb 27
TI  - Treatment of streptozotocin-induced diabetes mellitus by transplantation of islet 
      cells plus bone marrow cells via portal vein in rats.
PG  - 512-8
AB  - BACKGROUND: We have established a new method for the transplantation of 
      allogeneic pancreatic islets (PIs) using sublethal irradiation (9 Gy) plus 
      simultaneous transplantation of PIs and bone marrow cells (BMCs) via the portal 
      vein (PV) followed by intravenous (i.v.) injection of donor BMCs (9 Gy + PV + 
      i.v.). METHODS: Approximately 600 PIs of Brown Norway (BN: RT1An, RT1Bn) rats 
      were transplanted into the liver of streptozotocin-induced diabetic Fischer 344 
      (F344: RT1Al, RT1Bl) rats via the PV. BMCs (3x108) of BN rats were injected via 
      the PV or i.v. into the recipients simultaneously. In some groups, additional 
      i.v. injections of BMCs from BN rats were given 5 days after the PI 
      transplantation. RESULTS: All the recipients (10 of 10) in the 9 Gy + PV + i.v. 
      group showed normoglycemia for more than 1 year, whereas PIs were rejected within 
      30 days after transplantation in the group of 9 Gy + i.v. + i.v. CONCLUSIONS: 
      These results suggest that simultaneous transplantation of PIs and BMCs via the 
      PV is effective in inducing persistent tolerance.
FAU - Ikebukuro, Kazuya
AU  - Ikebukuro K
AD  - First Department of Pathology, Kansai Medical University, Osaka 570-8506, Japan.
FAU - Adachi, Yasushi
AU  - Adachi Y
FAU - Yamada, Yuichiro
AU  - Yamada Y
FAU - Fujimoto, Shimpei
AU  - Fujimoto S
FAU - Seino, Yutaka
AU  - Seino Y
FAU - Oyaizu, Haruki
AU  - Oyaizu H
FAU - Hioki, Koshiro
AU  - Hioki K
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Blood Glucose)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Bone Marrow Transplantation/methods/*physiology
MH  - Diabetes Mellitus, Experimental/*surgery
MH  - Female
MH  - Flow Cytometry
MH  - Graft Rejection/pathology
MH  - Graft Survival/immunology/*physiology
MH  - Immunosuppression Therapy/methods
MH  - Islets of Langerhans Transplantation/immunology/pathology/*physiology
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - Portal Vein
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred F344
MH  - T-Lymphocytes, Cytotoxic
MH  - Time Factors
EDAT- 2002/03/13 10:00
MHDA- 2002/04/03 10:01
CRDT- 2002/03/13 10:00
PHST- 2002/03/13 10:00 [pubmed]
PHST- 2002/04/03 10:01 [medline]
PHST- 2002/03/13 10:00 [entrez]
AID - 10.1097/00007890-200202270-00004 [doi]
PST - ppublish
SO  - Transplantation. 2002 Feb 27;73(4):512-8. doi: 10.1097/00007890-200202270-00004.