PMID- 11891962
OWN - NLM
STAT- MEDLINE
DCOM- 20020412
LR  - 20190922
IS  - 1043-3074 (Print)
IS  - 1043-3074 (Linking)
VI  - 24
IP  - 3
DP  - 2002 Mar
TI  - Genomic instability measurement in the diagnosis of thyroid neoplasms.
PG  - 290-5
AB  - BACKGROUND: Clinically palpable thyroid nodules are present in approximately 10% 
      of the population, although only 5% to 7% of these nodules harbor malignancy. 
      Fine-needle aspiration has become one of the central tools in the diagnostic 
      armamentarium of the surgeon/endocrinologist. There is, however, up to a 30% 
      indeterminate diagnostic rate associated with this technique, resulting in 
      unnecessary surgical interventions for patients harboring benign disease. A 
      second issue of clinical importance is the unreliability of predicting outcomes 
      based either on histologic findings alone or in combination with clinical 
      staging. To address these diagnostic and clinical shortcomings, we have used 
      measurement of genomic instability as a diagnostic and prognostic indicator for 
      thyroid neoplasms. METHODS: Genomic instability of thyroid tissue samples was 
      determined by inter-(simple sequence repeat) PCR, microsatellite instability 
      analysis, and fluorescence in situ hybridization (FISH) on thyroid neoplasms from 
      22 patients. RESULTS: Inter-(simple sequence repeat) PCR detected genomic 
      instability with an index range 0% to 1.9% (mean, 0.56%) in patients with benign 
      disease, whereas in patients with malignant histologic findings the values ranged 
      from 0% to 6.6% (mean, 2.9%). This difference between benign and malignant values 
      was statistically significant (p =.004). There was no demonstrable microsatellite 
      instability or loss of heterozygosity for six markers examined in this group. 
      Losses of chromosomes 17 and X in benign disease and gains of chromosomes 7, 12, 
      17, and X in Hurthle cell carcinoma were observed, although not at a significant 
      rate. CONCLUSIONS: Genomic instability as measured by inter-(simple sequence 
      repeat) PCR was significantly higher for malignant diseases compared with benign 
      thyroid tissues, but no such association was seen with aneuploidy or 
      microsatellite instability.
CI  - Copyright 2002 Wiley Periodicals, Inc.
FAU - Stoler, Daniel L
AU  - Stoler DL
AD  - Department of Experimental Pathology, Roswell Park Cancer Institute, Buffalo, New 
      York, USA.
FAU - Datta, Rajiv V
AU  - Datta RV
FAU - Charles, Melita A
AU  - Charles MA
FAU - Block, AnneMarie W
AU  - Block AW
FAU - Brenner, Bruce M
AU  - Brenner BM
FAU - Sieczka, Elizabeth M
AU  - Sieczka EM
FAU - Hicks, Wesley L Jr
AU  - Hicks WL Jr
FAU - Loree, Thom R
AU  - Loree TR
FAU - Anderson, Garth R
AU  - Anderson GR
LA  - eng
GR  - CA16056/CA/NCI NIH HHS/United States
GR  - CA74127/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Head Neck
JT  - Head & neck
JID - 8902541
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aneuploidy
MH  - Carcinoma/diagnosis/*genetics
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Loss of Heterozygosity
MH  - Male
MH  - Microsatellite Repeats/*genetics
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Thyroid Neoplasms/diagnosis/*genetics
EDAT- 2002/03/14 10:00
MHDA- 2002/04/16 10:01
CRDT- 2002/03/14 10:00
PHST- 2002/03/14 10:00 [pubmed]
PHST- 2002/04/16 10:01 [medline]
PHST- 2002/03/14 10:00 [entrez]
AID - 10.1002/hed.10050 [pii]
AID - 10.1002/hed.10050 [doi]
PST - ppublish
SO  - Head Neck. 2002 Mar;24(3):290-5. doi: 10.1002/hed.10050.