PMID- 11892748
OWN - NASA
STAT- MEDLINE
DCOM- 20020322
LR  - 20061115
IS  - 1002-0837 (Print)
IS  - 1002-0837 (Linking)
VI  - 13
IP  - 4
DP  - 2000 Aug
TI  - [A study of apoptosis and related gene bcl-2 and p53 expression in hippocampus of 
      rats exposed to repeated +Gz].
PG  - 263-6
AB  - Objective. To investigate the role of apoptosis in mechanisms of brain damage 
      induced by +Gz exposures. Method. Twenty conscious SD rats were randomly divided 
      into 5 groups. Rats in the control group (n=4) were exposed to +1 Gz and rats in 
      the 4 experimental groups (n=16) were exposed to +14 Gz for three times, each for 
      45 seconds with 30 min interval in between. All the +Gz exposured were on an 
      animal centrifuge. The rat brains were taken 30 min, 6 h, 24 h and 48 h after the 
      last centrifuge run and fixed and embedded. The apoptosis and expression changes 
      of related gene bcl-2 and p53 were detected by terminal deoxynucleotide 
      (correction of deoxynuleotide) transferase-mediated dUTP nick end labeling 
      (TUNEL) technique and immunohistochemical method, respectively. Result. Apoptotic 
      cells and expression changes of bcl-2 and p53 were observed in CA1 subregion of 
      rat hippocampus taken 6 h after repeated +Gz exposures, but returned to normal 
      after 24-48 h. Conclusion. It suggests that apoptosis and expression changes of 
      bcl-2 and p53 in rats hippocampus can be induced by repeated +Gz exposures and 
      the apoptosis is one of the molecular mechanisms of brain damage induced by 
      repeated +Gz exposures.
FAU - Cai, Q
AU  - Cai Q
AD  - Department of Molecular Biology, General Hospital of Air Force, Beijing, China.
FAU - Liu, H J
AU  - Liu HJ
FAU - Zhan, Z
AU  - Zhan Z
FAU - Zhu, M C
AU  - Zhu MC
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Space Med Med Eng (Beijing)
JT  - Hang tian yi xue yu yi xue gong cheng = Space medicine & medical engineering
JID - 9425305
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Brain Injuries/genetics/physiopathology
MH  - Centrifugation
MH  - Gene Expression
MH  - *Genes, bcl-2
MH  - *Genes, p53
MH  - Hippocampus/cytology/*metabolism/physiopathology
MH  - *Hypergravity
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
OID - NASA: 00027412
EDAT- 2002/03/15 10:00
MHDA- 2002/03/23 10:01
CRDT- 2002/03/15 10:00
PHST- 2002/03/15 10:00 [pubmed]
PHST- 2002/03/23 10:01 [medline]
PHST- 2002/03/15 10:00 [entrez]
PST - ppublish
SO  - Space Med Med Eng (Beijing). 2000 Aug;13(4):263-6.