PMID- 11893012
OWN - NLM
STAT- MEDLINE
DCOM- 20020911
LR  - 20190515
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 19
IP  - 1
DP  - 2002 Jan
TI  - Expression and function of 3beta hydroxisteroid dehydrogenase (3beta HSD) type II 
      and corticosteroid binding globulin (CBG) in granulosa cells from ovaries of 
      women with and without endometriosis.
PG  - 24-30
AB  - PURPOSE: To investigate the secretion of progesterone (P4) and corticosteroid 
      binding globulin (CBG) by granulosa luteal cells (GC) as well as the mRNA levels 
      of CBG and 3beta hydroxisteroid dehydrogenase (3beta HSD), in women with and 
      without endometriosis in vivo and in vitro. METHODS: Prospective study in a 
      private, university-affiliated assisted reproduction unit, including women with 
      severe endometriosis (n = 14) or without the disease (n = 20) undergoing in vitro 
      fertilization/intracytoplasmic sperm injection and embryo transfer. GC were 
      obtained from each follicle aspirated, pooled for each patient, and follicular 
      and blood contaminating leukocytes depleted through immunomagnetic purification. 
      Secreted P4 and CBG, and mRNA for both 3beta HSD and CBG were determined in vivo 
      and in vitro using RIA and reverse transcription followed by competitive 
      polymerase chain reaction (cRT-PCR). RESULTS: The pattern of expression of 3beta 
      HSD and CBG mRNAs in vivo and in vitro was similar in both groups. Also, GC from 
      patients with endometriosis produced equal amounts of P4 and CBG than controls 
      without the disease, either in freshly isolated cells or in 24-h cultures. 
      CONCLUSIONS: The GC function in terms of 3beta HSD and CBG mRNA expression and 
      P4/CBG secretion does not seem to be altered in patients with endometriosis in 
      comparison with those without this condition.
FAU - Garrido, Nicolas
AU  - Garrido N
AD  - Fundacion Instituto Valenciano de Infertilidad para el Estudio de la 
      Reproduccion, Valencia, Spain.
FAU - Krussel, Jan S
AU  - Krussel JS
FAU - Remohi, Jose
AU  - Remohi J
FAU - Simon, Carlos
AU  - Simon C
FAU - Pellicer, Antonio
AU  - Pellicer A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 9010-38-2 (Transcortin)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/*metabolism
MH  - Endometriosis/*metabolism/pathology
MH  - Female
MH  - Follicular Fluid/metabolism
MH  - Granulosa Cells/*metabolism
MH  - Humans
MH  - Prospective Studies
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcortin/*metabolism
PMC - PMC3455673
EDAT- 2002/03/15 10:00
MHDA- 2002/09/12 10:01
PMCR- 2003/01/01
CRDT- 2002/03/15 10:00
PHST- 2002/03/15 10:00 [pubmed]
PHST- 2002/09/12 10:01 [medline]
PHST- 2002/03/15 10:00 [entrez]
PHST- 2003/01/01 00:00 [pmc-release]
AID - 364835 [pii]
AID - 10.1023/a:1014058622697 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2002 Jan;19(1):24-30. doi: 10.1023/a:1014058622697.