PMID- 11895781
OWN - NLM
STAT- MEDLINE
DCOM- 20020502
LR  - 20211203
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 99
IP  - 7
DP  - 2002 Apr 1
TI  - CD4(+) and CD8(+) anergic T cells induced by interleukin-10-treated human 
      dendritic cells display antigen-specific suppressor activity.
PG  - 2468-76
AB  - Interleukin-10 (IL-10)-treated dendritic cells (DCs) induce an alloantigen- or 
      peptide-specific anergy in various CD4(+) and CD8(+) T-cell populations. In the 
      present study, we analyzed whether these anergic T cells are able to regulate 
      antigen-specific immunity. Coculture experiments revealed that 
      alloantigen-specific anergic CD4(+) and CD8(+) T cells suppressed proliferation 
      of syngeneic T cells in a dose-dependent manner. The same effect was observed 
      when the hemagglutinin-specific CD4(+) T-cell clone HA1.7 or tyrosinase-specific 
      CD8(+) T cells were cocultured with anergic T cells of the same specificity. 
      Anergic T cells did not induce an antigen-independent bystander inhibition. 
      Suppression was dependent on cell-to-cell contact between anergic and responder T 
      cells, required activation by antigen-loaded DCs, and was not mediated by 
      supernatants of anergic T cells. Furthermore, anergic T cells displayed an 
      increased extracellular and intracellular expression of cytotoxic T-lymphocyte 
      antigen (CTLA)-4 molecules, and blocking of the CTLA-4 pathway restored the 
      T-cell proliferation up to 70%, indicating an important role of the CTLA-4 
      molecule in the suppressor activity of anergic T cells. Taken together, our 
      experiments demonstrate that anergic T cells induced by IL-10-treated DCs are 
      able to suppress activation and function of T cells in an antigen-specific 
      manner. Induction of anergic T cells might be exploited therapeutically for 
      suppression of cellular immune responses in allergic or autoimmune diseases with 
      identified (auto) antigens.
FAU - Steinbrink, Kerstin
AU  - Steinbrink K
AD  - Department of Dermatology, University of Mainz, Langenbeckstrasse 1, D-551312 
      Mainz, Germany. steinbrink@hautklinik.klinik.uni-mainz.de
FAU - Graulich, Edith
AU  - Graulich E
FAU - Kubsch, Sebastian
AU  - Kubsch S
FAU - Knop, Jurgen
AU  - Knop J
FAU - Enk, Alexander H
AU  - Enk AH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CD4 Antigens)
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (CTLA4 protein, human)
RN  - 0 (Immunoconjugates)
RN  - 0 (Isoantigens)
RN  - 130068-27-8 (Interleukin-10)
RN  - 7D0YB67S97 (Abatacept)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
SB  - IM
MH  - Abatacept
MH  - Antigen-Presenting Cells/immunology
MH  - Antigens, CD
MH  - Antigens, Differentiation/immunology
MH  - Antigens, Neoplasm/analysis
MH  - CD4 Antigens/analysis
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - CTLA-4 Antigen
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Clonal Anergy/*immunology
MH  - Coculture Techniques
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Humans
MH  - *Immunoconjugates
MH  - Immunosuppression Therapy
MH  - Interleukin-10/*pharmacology
MH  - Isoantigens/immunology
MH  - Leukapheresis
MH  - Leukocytes, Mononuclear/cytology/immunology
MH  - Lymphocyte Activation
MH  - Melanoma/immunology
MH  - Monophenol Monooxygenase/analysis
MH  - T-Lymphocytes, Regulatory/immunology
EDAT- 2002/03/16 10:00
MHDA- 2002/05/03 10:01
CRDT- 2002/03/16 10:00
PHST- 2002/03/16 10:00 [pubmed]
PHST- 2002/05/03 10:01 [medline]
PHST- 2002/03/16 10:00 [entrez]
AID - S0006-4971(20)38034-4 [pii]
AID - 10.1182/blood.v99.7.2468 [doi]
PST - ppublish
SO  - Blood. 2002 Apr 1;99(7):2468-76. doi: 10.1182/blood.v99.7.2468.