PMID- 11895937
OWN - NLM
STAT- MEDLINE
DCOM- 20020411
LR  - 20220409
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 70
IP  - 4
DP  - 2002 Apr
TI  - Identification of motility and autoagglutination Campylobacter jejuni mutants by 
      random transposon mutagenesis.
PG  - 1761-71
AB  - Campylobacter jejuni has been identified as the leading cause of acute bacterial 
      diarrhea in the United States, yet compared with other enteric pathogens, 
      considerably less is understood concerning the virulence factors of this human 
      pathogen. A random in vivo transposon mutagenesis system was recently developed 
      for the purpose of creating a library of C. jejuni transformants. A total of 
      1,065 C. jejuni transposon mutants were screened for their ability to swarm on 
      motility agar plates and autoagglutinate in liquid cultures; 28 mutants were 
      subsequently identified. The transposon insertion sites were obtained by using 
      random-primed PCR, and the putative genes responsible for these phenotypes were 
      identified. Of these mutants, all 28 were found to have diminished motility (0 to 
      86% that of the control). Seventeen motility mutants had insertions in genes with 
      strong homology to functionally known motility and chemotaxis genes; however, 11 
      insertions were in genes of unknown function. Twenty motility mutants were unable 
      to autoagglutinate, suggesting that the expression of flagella is correlated with 
      autoagglutination (AAG). However, four mutants expressed wild-type levels of 
      surface FlaA, as indicated by Western blot analysis, yet were unable to 
      autoagglutinate (Cj1318, Cj1333, Cj1340c, and Cj1062). These results suggest that 
      FlaA is necessary but not sufficient to mediate the AAG phenotype. Furthermore, 
      two of the four AAG mutants (Cj1333 and Cj1062) were unable to invade INT-407 
      intestinal epithelial cells, as determined by a gentamicin treatment assay. These 
      data identify novel genes important for motility, chemotaxis, and AAG and 
      demonstrate their potential role in virulence.
FAU - Golden, Neal J
AU  - Golden NJ
AD  - Department of Epidemiology and Preventative Medicine, University of Maryland 
      School of Medicine, Baltimore, Maryland 21201, USA.
FAU - Acheson, David W K
AU  - Acheson DW
LA  - eng
GR  - AI-16242/AI/NIAID NIH HHS/United States
GR  - AI-39067/AI/NIAID NIH HHS/United States
GR  - HL-55660/HL/NHLBI NIH HHS/United States
GR  - P30 DK-34928/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (DNA Transposable Elements)
RN  - 12777-81-0 (Flagellin)
RN  - 133606-66-3 (flaA protein, bacteria)
SB  - IM
MH  - *Agglutination
MH  - Bacterial Adhesion
MH  - Campylobacter jejuni/*genetics/pathogenicity/physiology
MH  - Cell Line
MH  - *DNA Transposable Elements
MH  - Flagellin/analysis
MH  - Intestinal Mucosa/microbiology
MH  - Microscopy, Fluorescence
MH  - Movement
MH  - Mutagenesis
MH  - Mutation
PMC - PMC127829
EDAT- 2002/03/16 10:00
MHDA- 2002/04/12 10:01
PMCR- 2002/04/01
CRDT- 2002/03/16 10:00
PHST- 2002/03/16 10:00 [pubmed]
PHST- 2002/04/12 10:01 [medline]
PHST- 2002/03/16 10:00 [entrez]
PHST- 2002/04/01 00:00 [pmc-release]
AID - 0990 [pii]
AID - 10.1128/IAI.70.4.1761-1771.2002 [doi]
PST - ppublish
SO  - Infect Immun. 2002 Apr;70(4):1761-71. doi: 10.1128/IAI.70.4.1761-1771.2002.