PMID- 11896155
OWN - NLM
STAT- MEDLINE
DCOM- 20020327
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 22
IP  - 6
DP  - 2002 Mar 15
TI  - Rapid synaptic remodeling by protein kinase C: reciprocal translocation of NMDA 
      receptors and calcium/calmodulin-dependent kinase II.
PG  - 2153-64
AB  - In contrast to the rapid regulation of AMPA receptors, previous evidence has 
      supported the idea that the synaptic density of NMDA-type glutamate receptors is 
      fairly static, modulated only over a long time scale in a homeostatic manner. We 
      report here that selective activation of protein kinase C (PKC) with phorbol 
      esters induces a rapid dispersal of NMDA receptors from synaptic to extrasynaptic 
      plasma membrane in cultured rat hippocampal neurons. PKC activation induced a 
      simultaneous translocation of calcium/calmodulin-dependent kinase II (CaMKII) to 
      synapses but no change in spine number, presynaptic terminal number, or the 
      distribution of AMPA receptors or the synaptic scaffolding protein PSD-95. 
      PKC-induced accumulation of CaMKII was dependent on filamentous actin, whereas 
      dispersal of NMDA receptors occurred by a different mechanism independent of 
      actin or CaMKII. Consistent with the decrease in synaptic density of NMDA 
      receptors, phorbol ester pretreatment reduced excitotoxicity. These results 
      reveal a surprisingly dynamic nature to the molecular composition and functional 
      properties of glutamatergic postsynaptic specializations.
FAU - Fong, Dan K
AU  - Fong DK
AD  - Department of Anatomy and Neurobiology, Washington University School of Medicine, 
      St. Louis, Missouri 63110, USA.
FAU - Rao, Anuradha
AU  - Rao A
FAU - Crump, F Thomas
AU  - Crump FT
FAU - Craig, Ann Marie
AU  - Craig AM
LA  - eng
GR  - R01 NS033184/NS/NINDS NIH HHS/United States
GR  - NS33184/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (NR1 NMDA receptor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phorbol Esters)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (postsynaptic density proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - Cytoskeleton/metabolism
MH  - Dendrites/metabolism/ultrastructure
MH  - Disks Large Homolog 4 Protein
MH  - Enzyme Activation/drug effects
MH  - Gene Expression
MH  - Green Fluorescent Proteins
MH  - Intracellular Signaling Peptides and Proteins
MH  - Luminescent Proteins/genetics
MH  - Membrane Proteins
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/cytology/drug effects/metabolism
MH  - Phorbol Esters/pharmacology
MH  - Protein Kinase C/*metabolism
MH  - Protein Transport/drug effects/physiology
MH  - Rats
MH  - Receptors, AMPA/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Synapses/*metabolism
MH  - Time Factors
PMC - PMC6758278
EDAT- 2002/03/16 10:00
MHDA- 2002/03/28 10:01
PMCR- 2002/09/15
CRDT- 2002/03/16 10:00
PHST- 2002/03/16 10:00 [pubmed]
PHST- 2002/03/28 10:01 [medline]
PHST- 2002/03/16 10:00 [entrez]
PHST- 2002/09/15 00:00 [pmc-release]
AID - 22/6/2153 [pii]
AID - 6176 [pii]
AID - 10.1523/JNEUROSCI.22-06-02153.2002 [doi]
PST - ppublish
SO  - J Neurosci. 2002 Mar 15;22(6):2153-64. doi: 10.1523/JNEUROSCI.22-06-02153.2002.