PMID- 11896289
OWN - NLM
STAT- MEDLINE
DCOM- 20020621
LR  - 20190513
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 66
IP  - 2
DP  - 2002 Apr
TI  - Ranking of allergenic potency of rubber chemicals in a modified local lymph node 
      assay.
PG  - 226-32
AB  - A modified local lymph node assay (LLNA) with ex vivo tritium thymidine (3H-TdR) 
      labeling of the proliferating lymph node cells was used for determination of the 
      allergenic potency of chemicals used in the production of rubber for latex 
      medical gloves. Fifteen chemicals known to induce contact hypersensitivity 
      reactions in man, including various thiuram, carbamate, and benzothiazole 
      compounds, and one amine were tested. The EC3 (effective concentration inducing a 
      3-fold increase in proliferation of lymph node cells [Stimulation Index, SI = 3]) 
      was calculated with nonlinear regression analysis, including a bootstrap method 
      for determination of the 5-95% confidence interval of the EC3 value. This 
      procedure identified 14 out of the 15 chemicals tested as sensitizers, while for 
      one chemical, ZDBC, no EC3 could be calculated due to low responses and a lack of 
      a dose-response relationship in the data obtained. The ranking order of the 
      chemicals with increasing EC3 values (and thus decreasing allergenic potency) was 
      found to be in the following order: ZDEC < TMTD < TETD < ZPC < ZDMC < MBTS < PTD 
      < TMTM < MBT < MBI < PTT < ZMBT < TBTD < DEA < ZDBC. Our results indicate that 
      the chemicals of choice for use in the production of natural rubber latex 
      products would be for the thiuram compounds, TBTD; for the carbamates, ZDBC; and 
      for the benzothiazoles, ZMBT. However, one has to be aware that besides potency, 
      the total amount of residual chemical present in the final product is also 
      important for allergy induction.
FAU - De Jong, Wim H
AU  - De Jong WH
AD  - Laboratory for Pathology and Immunobiology, National Institute for Public Health 
      and the Environment, RIVM, P.O.Box 1, 3720 BA Bilthoven, The Netherlands. 
      w.de.jong@rivm.nl
FAU - Van Och, Francois M M
AU  - Van Och FM
FAU - Den Hartog Jager, Constance F
AU  - Den Hartog Jager CF
FAU - Spiekstra, Sander W
AU  - Spiekstra SW
FAU - Slob, W
AU  - Slob W
FAU - Vandebriel, Rob J
AU  - Vandebriel RJ
FAU - Van Loveren, Henk
AU  - Van Loveren H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Allergens)
SB  - IM
MH  - Administration, Cutaneous
MH  - Allergens/classification/*toxicity
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Latex Hypersensitivity/*etiology
MH  - Local Lymph Node Assay
MH  - Lymph Nodes/*drug effects/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
EDAT- 2002/03/16 10:00
MHDA- 2002/06/22 10:01
CRDT- 2002/03/16 10:00
PHST- 2002/03/16 10:00 [pubmed]
PHST- 2002/06/22 10:01 [medline]
PHST- 2002/03/16 10:00 [entrez]
AID - 10.1093/toxsci/66.2.226 [doi]
PST - ppublish
SO  - Toxicol Sci. 2002 Apr;66(2):226-32. doi: 10.1093/toxsci/66.2.226.