PMID- 11897004
OWN - NLM
STAT- MEDLINE
DCOM- 20020819
LR  - 20191105
IS  - 0908-665X (Print)
IS  - 0908-665X (Linking)
VI  - 9
IP  - 2
DP  - 2002 Mar
TI  - Expression of xenogeneic MHC class II molecules in HLA-DR(+) and -DR(-) cells: 
      influence of retrovirus vector design and cellular context.
PG  - 115-24
AB  - We recently established that molecular chimeras of major histocompatibility 
      complex (MHC) class II molecules, created via retroviral transfer of allogeneic 
      class II cDNAs into bone marrow cells (BMCs), alleviated complications associated 
      with mixed BMC chimeras while leading to T cell tolerance to renal grafts sharing 
      the transferred class II. Initially demonstrated for allogeneic transplants in 
      miniature swine, this concept was extended to T-dependent antibody (Ab) responses 
      to xenogeneic antigens (Ags) in the pig --> baboon combination. Successful 
      down-regulation of T cell responses appeared, however, to be contingent on a 
      tight lineage-specific expression of transferred class II molecules. The present 
      studies were, therefore, designed to evaluate the influence of construct design 
      and cellular environment on expression of retrovirally transferred xenogeneic 
      class II cDNAs. Proviral genomes for pig class II SLA-DR expression, differing 
      only at the marker neo(r) or enhanced green fluorescent protein (EGFP) gene, 
      showed increased membrane SLA-DR density on HLA-DR(-) fibroblasts as well as 
      HLA-DR(+), TF-1 erythroleukemia cells. More importantly, HLA-DR(+) human B cell 
      lines, although efficiently transduced with pig DR retroviruses, exhibited 
      unstable surface pig DR. Surface pig DR- B cells, nevertheless, stimulated 
      autologous human T cells pre-sensitized to pig Ags, a proliferation likely 
      occurring through presentation of class II-derived peptides. Collectively, these 
      data suggest that surface expression of transferred class II molecules is not 
      related to the ability of recipient cells to synthesize xenogeneic class II 
      molecules but rather to their Ag processing capacities.
FAU - Denaro, Maria
AU  - Denaro M
AD  - BioTransplant Incorporated, Charlestown Navy Yard, Charlestown, MA, USA.
FAU - Kolber-Simonds, Donna
AU  - Kolber-Simonds D
FAU - Schad, Victoria
AU  - Schad V
FAU - Muthukumar, Shanthini
AU  - Muthukumar S
FAU - Germana, Sharon
AU  - Germana S
FAU - White-Scharf, Mary E
AU  - White-Scharf ME
FAU - Banerjee, Papia T
AU  - Banerjee PT
FAU - LeGuern, Christian
AU  - LeGuern C
FAU - Andersson, Goran
AU  - Andersson G
LA  - eng
GR  - 2R01-AI33053/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Denmark
TA  - Xenotransplantation
JT  - Xenotransplantation
JID - 9438793
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cell Line
MH  - Genetic Vectors
MH  - Graft Rejection/*genetics/*immunology/prevention & control
MH  - HLA-DR Antigens/genetics/immunology
MH  - Histocompatibility Antigens Class II/*genetics/immunology
MH  - Humans
MH  - Organ Transplantation
MH  - Papio
MH  - Retroviridae
MH  - Swine
MH  - Transgenes
MH  - Transplantation Immunology
MH  - Transplantation, Heterologous
EDAT- 2002/03/19 10:00
MHDA- 2002/08/20 10:01
CRDT- 2002/03/19 10:00
PHST- 2002/03/19 10:00 [pubmed]
PHST- 2002/08/20 10:01 [medline]
PHST- 2002/03/19 10:00 [entrez]
AID - 1o038 [pii]
AID - 10.1034/j.1399-3089.2002.1o038.x [doi]
PST - ppublish
SO  - Xenotransplantation. 2002 Mar;9(2):115-24. doi: 10.1034/j.1399-3089.2002.1o038.x.