PMID- 11897026
OWN - NLM
STAT- MEDLINE
DCOM- 20020701
LR  - 20211004
IS  - 1474-760X (Electronic)
IS  - 1465-6906 (Print)
IS  - 1474-7596 (Linking)
VI  - 3
IP  - 3
DP  - 2002
TI  - Molecular genetics and structural genomics of the human protein kinase C gene 
      module.
PG  - RESEARCH0014
AB  - BACKGROUND: Protein kinase C (PKC) has become a major focus among cell biologists 
      interested in second-messenger signal transduction and much has been learned 
      about differences in the cellular localization and function of its different 
      isotypes. In this study we systematically address the genomic locations and gene 
      structures of the human PKC gene module. RESULTS: We first carried out fine 
      chromosomal mapping of all nine PKC genes by fluorescence in situ hybridization 
      (FISH), using cosmid and BAC probes. The PKC genes are found to be dispersed 
      throughout the genome, and in some positions distinct from those previously 
      reported: PKCalpha is at 17q24, PKCbeta at 16p12, PKCgamma at 19q13.4, PKCdelta 
      at 3p21.2, PKCepsilon at 2p21, PKCzeta at 1p36.3, PKCeta at 14q22-23, PKCtheta; 
      at 10p15 and PKCiota at 3q26. For PKCiota, an additional FISH signal mapped on 
      Xq21.3 revealed a pseudogene (derived by retrotransposition). PKCgamma, zeta, and 
      theta; are found to map to the most distal positions on the chromosomes, 
      potentially implicating telomere position effects in their expression. Using the 
      complete human genome draft sequence and bioinformatics tools, we then carried 
      out a systematic analysis of PKC gene structure, including determination of the 
      occurrence of single-nucleotide polymorphisms corresponding to the PKC loci. 
      CONCLUSION: This resource of genomic information now facilitates investigation of 
      the PKC gene module in structural chromosomal abnormalities and human disease 
      locus mapping studies.
FAU - Kofler, Kurt
AU  - Kofler K
AD  - Institute for Medical Biology and Human Genetics, University of Innsbruck, 
      Schoepfstrasse 41, A-6020 Innsbruck, Austria. Gottfried.Baier@uibk.ac.at
FAU - Erdel, Martin
AU  - Erdel M
FAU - Utermann, Gerd
AU  - Utermann G
FAU - Baier, Gottfried
AU  - Baier G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020227
PL  - England
TA  - Genome Biol
JT  - Genome biology
JID - 100960660
RN  - 0 (Genetic Markers)
RN  - 0 (Isoenzymes)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Alternative Splicing/genetics
MH  - Animals
MH  - Chromosome Mapping/methods
MH  - Genetic Markers/genetics
MH  - Genomics/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Isoenzymes/genetics
MH  - Mice
MH  - Polymorphism, Single Nucleotide/genetics
MH  - Protein Kinase C/*genetics
MH  - Rats
PMC - PMC88812
EDAT- 2002/03/19 10:00
MHDA- 2002/07/02 10:01
PMCR- 2002/02/27
CRDT- 2002/03/19 10:00
PHST- 2002/01/02 00:00 [received]
PHST- 2002/02/05 00:00 [revised]
PHST- 2002/02/06 00:00 [accepted]
PHST- 2002/03/19 10:00 [pubmed]
PHST- 2002/07/02 10:01 [medline]
PHST- 2002/03/19 10:00 [entrez]
PHST- 2002/02/27 00:00 [pmc-release]
AID - gb-2002-3-3-research0014 [pii]
AID - 10.1186/gb-2002-3-3-research0014 [doi]
PST - ppublish
SO  - Genome Biol. 2002;3(3):RESEARCH0014. doi: 10.1186/gb-2002-3-3-research0014. Epub 
      2002 Feb 27.