PMID- 11897492
OWN - NLM
STAT- MEDLINE
DCOM- 20020531
LR  - 20191210
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 14
IP  - 6
DP  - 2002 Jun
TI  - Luminal Ca(2+) and the activity of sarcoplasmic reticulum Ca(2+) pumps modulate 
      histamine-induced all-or-none Ca(2+) release in smooth muscle cells.
PG  - 517-27
AB  - We have studied histamine (HA)-evoked intracellular Ca(2+) release in single, 
      freshly isolated myocytes from the guinea pig urinary bladder. Short applications 
      of histamine (5 s) produced a thapsigargin (TG)-sensitive transient increase in 
      intracellular calcium concentration ([Ca(2+)](i)). It was established that 
      histamine and caffeine (Caff) released Ca(2+) from the same intracellular stores 
      in these cells. Reducing the Ca(2+) content of internal stores by incubating 
      cells with U-73343 or cyclopiazonic acid (CPA) inhibited the histamine-evoked 
      Ca(2+) release in 69% and 60% of cells, respectively. Under these conditions, all 
      cells released Ca(2+) in response to either caffeine or acetylcholine (ACh). 
      However, decreasing internal Ca(2+) stores by removing external Ca(2+) inhibited 
      histamine-induced Ca(2+) mobilization in only 22% of cells. A similar small 
      fraction of cells was inhibited when sarcoplasmic reticulum (SR) Ca(2+) pumps 
      were quickly blocked to avoid a significant reduction of luminal Ca(2+). In 
      conclusion, lowering the luminal Ca(2+) content in combination with an impairment 
      of the SR Ca(2+) pump activity significantly diminishes the ability of histamine 
      to evoke an all-or-none intracellular Ca(2+) release.
FAU - Rueda, Angelica
AU  - Rueda A
AD  - Departamento de Bioquimica, CINVESTAV-IPN, Apdo. Postal 14-740, Mexico, D.F. 
      07000, Mexico.
FAU - Garcia, Lucia
AU  - Garcia L
FAU - Guerrero-Hernandez, Agustin
AU  - Guerrero-Hernandez A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Estrenes)
RN  - 0 (Histamine Antagonists)
RN  - 0 (Indoles)
RN  - 0 (Pyrrolidinones)
RN  - 142878-12-4 (U 73343)
RN  - 820484N8I3 (Histamine)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - N9YNS0M02X (Acetylcholine)
RN  - SY7Q814VUP (Calcium)
RN  - X9TLY4580Z (cyclopiazonic acid)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Calcium/*metabolism/physiology
MH  - Calcium-Transporting ATPases/*physiology
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Estrenes/pharmacology
MH  - Guinea Pigs
MH  - Histamine/*pharmacology
MH  - Histamine Antagonists/pharmacology
MH  - Indoles/pharmacology
MH  - Inositol 1,4,5-Trisphosphate/biosynthesis
MH  - Kinetics
MH  - Male
MH  - Muscle, Smooth/drug effects/*metabolism
MH  - Pyrrolidinones/pharmacology
MH  - Sarcoplasmic Reticulum/drug effects/enzymology/*metabolism
MH  - Sarcoplasmic Reticulum Calcium-Transporting ATPases
MH  - Urinary Bladder/metabolism
EDAT- 2002/03/19 10:00
MHDA- 2002/06/01 10:01
CRDT- 2002/03/19 10:00
PHST- 2002/03/19 10:00 [pubmed]
PHST- 2002/06/01 10:01 [medline]
PHST- 2002/03/19 10:00 [entrez]
AID - S0898656801002844 [pii]
AID - 10.1016/s0898-6568(01)00284-4 [doi]
PST - ppublish
SO  - Cell Signal. 2002 Jun;14(6):517-27. doi: 10.1016/s0898-6568(01)00284-4.