PMID- 11897872
OWN - NLM
STAT- MEDLINE
DCOM- 20020621
LR  - 20061115
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 20
IP  - 2
DP  - 2002
TI  - Comparison of bone marrow cells harvested from various bones of cynomolgus 
      monkeys at various ages by perfusion or aspiration methods: a preclinical study 
      for human BMT.
PG  - 155-62
AB  - Using cynomolgus monkeys, we have previously established a new method for 
      harvesting bone marrow cells (BMCs) with minimal contamination of the BMCs with T 
      cells from the peripheral blood. We originally conducted this new "perfusion 
      method" in the long bones (the humerus, femur, and tibia) of cynomolgus monkeys. 
      Here, we apply the perfusion method to obtain BMCs from the ilium of cynomolgus 
      monkeys, since BMCs are usually collected from the ilium by the conventional 
      aspiration method in humans. The perfusion method consists of two approaches: 
      transverse iliac perfusion and longitudinal iliac perfusion. BMCs harvested by 
      the perfusion method from the long bones and ilium were compared with those 
      collected from the ilium by the aspiration method. The contamination of BMCs with 
      peripheral blood, determined by the frequencies of CD4+ and CD8+ T cells, was 
      significantly lower in BMCs obtained from the ilium or long bones by the 
      perfusion method (CD4+ plus CD8+ T cells <4%) than in those obtained by the iliac 
      aspiration method (CD4+ plus CD8+ T cells >20%). However, the numbers of immature 
      myeloid cells, such as myeloblasts, promyelocytes, myelocytes, and 
      metamyelocytes, were higher in BMCs obtained by the iliac perfusion method than 
      in those obtained by the iliac aspiration method. The assays for in vitro 
      colony-forming unit in culture revealed that progenitor activity was 
      significantly higher in BMCs obtained by the perfusion method than in those 
      obtained by the aspiration method. These findings suggest that the contamination 
      of BMCs with peripheral blood is much less when using the perfusion method than 
      when using the aspiration method. To determine the best site for harvesting BMCs 
      by the perfusion method, age-dependent changes in BMCs harvested by the perfusion 
      method from the long bones and ilium were examined. The numbers of BMCs varied in 
      the long bones (humerus > femur > tibia) and showed age-dependent decreases, 
      whereas they remained similar in the ilium of cynomolgus monkeys from 3 years to 
      6 years of age. However, in cynomolgus monkeys, BMC harvesting by the perfusion 
      method from the ilium (but not from the long bones) is found to involve the risk 
      of fat emboli, particularly when the BMCs are quickly perfused under high 
      pressure. These findings suggest, even in humans, that the perfusion method is 
      better than the aspiration method, and that the best site for collection of BMCs 
      is the humerus.
FAU - Kushida, Taketoshi
AU  - Kushida T
AD  - First Department of Pathology, Transplantation Center, Kansai Medical University, 
      10-15 Fumizono-cho, Moriguchi City, Osaka 570-8506, Japan.
FAU - Inaba, Muneo
AU  - Inaba M
FAU - Ikebukuro, Kazuya
AU  - Ikebukuro K
FAU - Ichioka, Naoya
AU  - Ichioka N
FAU - Esumi, Takashi
AU  - Esumi T
FAU - Oyaizu, Haruki
AU  - Oyaizu H
FAU - Yoshimura, Tomoo
AU  - Yoshimura T
FAU - Nagahama, Takashi
AU  - Nagahama T
FAU - Nakamura, Kouichi
AU  - Nakamura K
FAU - Ito, Tomoki
AU  - Ito T
FAU - Hisha, Hiroko
AU  - Hisha H
FAU - Sugiura, Kikuya
AU  - Sugiura K
FAU - Yasumizu, Ryoji
AU  - Yasumizu R
FAU - Iida, Hirokazu
AU  - Iida H
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Antigens, Surface)
RN  - 0 (Cytokines)
SB  - IM
MH  - Aging/metabolism/*pathology
MH  - Animals
MH  - Antigens, Surface/metabolism
MH  - Artifacts
MH  - Biopsy, Needle/adverse effects/*methods
MH  - Bone Marrow Cells/*cytology/immunology/metabolism
MH  - Bone Marrow Transplantation/*methods
MH  - Bone and Bones/*cytology/*surgery
MH  - Cytokines/metabolism
MH  - Femur/cytology/surgery
MH  - Humerus/cytology/surgery
MH  - Ilium/cytology/surgery
MH  - Macaca fascicularis
MH  - Myeloid Progenitor Cells/cytology/immunology/metabolism
MH  - Perfusion/adverse effects/*methods
MH  - Tissue and Organ Harvesting/*methods
EDAT- 2002/03/19 10:00
MHDA- 2002/06/22 10:01
CRDT- 2002/03/19 10:00
PHST- 2002/03/19 10:00 [pubmed]
PHST- 2002/06/22 10:01 [medline]
PHST- 2002/03/19 10:00 [entrez]
AID - 10.1634/stemcells.20-2-155 [doi]
PST - ppublish
SO  - Stem Cells. 2002;20(2):155-62. doi: 10.1634/stemcells.20-2-155.