PMID- 11900846
OWN - NLM
STAT- MEDLINE
DCOM- 20020712
LR  - 20190910
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 84
IP  - 1-2
DP  - 2002 Mar 20
TI  - Genetic characterization and phylogeny of human T-cell lymphotropic virus type I 
      from Chile.
PG  - 135-49
AB  - Infection with Human T-Cell Lymphotropic Virus type I (HTLV-I) have been 
      associated with the development of the HTLV-I associated myelopathy/tropical 
      spastic paraparesis (HAM/TSP). Phylogenetic analyses of HTLV-I isolates have 
      revealed that HTLV-I can be classified into three major groups: the Cosmopolitan, 
      Central African and Melanesian. In the present study, we analyzed the tax, 5' 
      ltr, gag, pol, and env sequences of proviruses of PBMC from ten HAM/TSP patients 
      to investigate the phylogenetic characterization of HTLV-I in Chilean patients. 
      HTLV-I provirus in PBMC from ten Chilean patients with HAM/TSP were amplified by 
      PCR using primers of tax, 5' ltr, gag, pol, and env genes. Amplified products of 
      the five genes were purified and nucleotide sequence was determined by the 
      dideoxy termination procedure. DNA sequences were aligned with the CLUSTAL W 
      program. The results of this study showed that the tax, 5' ltr, gag, pol, and env 
      gene of the Chilean HTLV-I strains had a nucleotide homology ranged from 98.1 to 
      100%, 95 to 97%, 98.9 to 100%, 94 to 98%, and 94.2 to 98.5% respect to ATK-1 
      clone, respectively. According to molecular phylogeny with 5' ltr gene, the 
      Chilean HTLV-I strains were grouped with each other suggesting one cluster 
      included in Transcontinental subgroup.
FAU - Ramirez, E
AU  - Ramirez E
AD  - Department of Virology, Public Health Institute of Chile, Av. Marathon 1000, 
      Santiago, Chile. eramirez@ispch.cl
FAU - Cartier, L
AU  - Cartier L
FAU - Villota, C
AU  - Villota C
FAU - Fernandez, J
AU  - Fernandez J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, tax)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chile
MH  - DNA, Viral
MH  - Female
MH  - Gene Products, tax/genetics
MH  - Genes, env
MH  - Genes, gag
MH  - Genes, pol
MH  - Human T-lymphotropic virus 1/classification/*genetics
MH  - Humans
MH  - Leukocytes, Mononuclear/virology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Paraparesis, Tropical Spastic/blood/*virology
MH  - Phylogeny
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Terminal Repeat Sequences
EDAT- 2002/03/20 10:00
MHDA- 2002/07/13 10:01
CRDT- 2002/03/20 10:00
PHST- 2002/03/20 10:00 [pubmed]
PHST- 2002/07/13 10:01 [medline]
PHST- 2002/03/20 10:00 [entrez]
AID - S0168170202000059 [pii]
AID - 10.1016/s0168-1702(02)00005-9 [doi]
PST - ppublish
SO  - Virus Res. 2002 Mar 20;84(1-2):135-49. doi: 10.1016/s0168-1702(02)00005-9.