PMID- 11901764
OWN - NLM
STAT- MEDLINE
DCOM- 20020412
LR  - 20051116
IS  - 1343-1420 (Print)
IS  - 1343-1420 (Linking)
VI  - 49
IP  - 1-2
DP  - 2002 Feb
TI  - Genetic modification of dendritic cells and its application for cancer 
      immunotherapy.
PG  - 7-17
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs). DCs 
      pulsed with peptides of tumor-associated antigens (TAA) and tumor lysate have 
      been used in cancer immunotherapy. An early clinical study demonstrated the 
      safety of the use of DCs, but the clinical response was not sufficient. The 
      gene-modification of DCs with TAA and soluble factor genes such as cytokine and 
      chemokine genes has been examined to enhance the antigen-presenting capacity of 
      DCs. Viral vectors including retroviruses and adenoviruses have been reported to 
      be useful to obtain a sufficient transduction efficiency into DCs. TAA 
      gene-transduced DCs could have several advantages compared with TAA 
      peptide-pulsed DCs as follows: 1) The use of TAA gene-modified DCs are not 
      restricted by MHC haplotypes. 2) The gene transduction with TAA genes is likely 
      to present the unknown TAA peptides on DCs. 3) The gene-modified DCs show the 
      prolonged presentation of TAA peptides. The transduction of DCs with cytokine 
      genes including IL-12 and GM-CSF have also been reported to argument the 
      antitumor effects of DCs. Although the results in the experimental systems were 
      promising, the clinical application of gene-modified DCs includes several 
      problems such as the standardization of methods of manipulation and 
      gene-transduction of DCs. Approaches to solve them require further studies.
FAU - Nishioka, Yasuhiko
AU  - Nishioka Y
AD  - Third Department of Internal Medicine, University of Tokushima School of 
      Medicine, Kuramoto-cho, Tokushima 770-8503, Japan.
FAU - Hua, Wen
AU  - Hua W
FAU - Nishimura, Naoki
AU  - Nishimura N
FAU - Sone, Saburo
AU  - Sone S
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - J Med Invest
JT  - The journal of medical investigation : JMI
JID - 9716841
SB  - IM
MH  - Animals
MH  - Dendritic Cells/chemistry/*metabolism/transplantation
MH  - Humans
MH  - Immunotherapy, Adoptive/*methods/trends
MH  - Neoplasms/*genetics/*therapy
RF  - 87
EDAT- 2002/03/21 10:00
MHDA- 2002/04/16 10:01
CRDT- 2002/03/21 10:00
PHST- 2002/03/21 10:00 [pubmed]
PHST- 2002/04/16 10:01 [medline]
PHST- 2002/03/21 10:00 [entrez]
PST - ppublish
SO  - J Med Invest. 2002 Feb;49(1-2):7-17.