PMID- 11901803
OWN - NLM
STAT- MEDLINE
DCOM- 20020426
LR  - 20101118
IS  - 1473-7159 (Print)
IS  - 1473-7159 (Linking)
VI  - 1
IP  - 1
DP  - 2001 May
TI  - Universal Linkage System: versatile nucleic acid labeling technique.
PG  - 81-91
AB  - Over the last two decades nonradioactive nucleic acid labeling and detection 
      systems have overcome the safety, disposal, stability and cost problems that are 
      associated with radioactive techniques. Besides traditional, enzyme-mediated, 
      nonradioactive labeling methods (e.g., random priming, nick translation or 
      labeling by PCR), several chemical labeling systems have been developed (e.g., 
      ULS, psoralen, alkylating agents). These methods provide fluorescent (or hapten) 
      labeled probes for fluorescence in situ hybridization (FISH), comparative genomic 
      hybridization (CGH) and microarray-based techniques. In this review the DNA-based 
      molecular diagnostic applications and perspectives of the Universal Linkage 
      System (ULS) technology will be described.
FAU - van Gijlswijk, R P
AU  - van Gijlswijk RP
AD  - Kreatech Diagnostics BV, PO Box 37078, 1030 AB Amsterdam, The Netherlands.
FAU - Talman, E G
AU  - Talman EG
FAU - Janssen, P J
AU  - Janssen PJ
FAU - Snoeijers, S S
AU  - Snoeijers SS
FAU - Killian, J
AU  - Killian J
FAU - Tanke, H J
AU  - Tanke HJ
FAU - Heetebrij, R J
AU  - Heetebrij RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Expert Rev Mol Diagn
JT  - Expert review of molecular diagnostics
JID - 101120777
RN  - 0 (DNA Probes)
RN  - 0 (Nucleic Acids)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Chlamydia trachomatis/genetics/isolation & purification
MH  - DNA Probes
MH  - Genetic Linkage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Molecular Diagnostic Techniques
MH  - Nucleic Acids/*chemistry
MH  - Oligonucleotide Array Sequence Analysis
MH  - Papillomaviridae/genetics/isolation & purification
MH  - RNA, Messenger
MH  - Staining and Labeling/*methods
MH  - Staphylococcus aureus/genetics/isolation & purification
RF  - 63
EDAT- 2002/03/21 10:00
MHDA- 2002/04/27 10:01
CRDT- 2002/03/21 10:00
PHST- 2002/03/21 10:00 [pubmed]
PHST- 2002/04/27 10:01 [medline]
PHST- 2002/03/21 10:00 [entrez]
AID - ERM010111 [pii]
AID - 10.1586/14737159.1.1.81 [doi]
PST - ppublish
SO  - Expert Rev Mol Diagn. 2001 May;1(1):81-91. doi: 10.1586/14737159.1.1.81.