PMID- 11905039
OWN - NLM
STAT- MEDLINE
DCOM- 20020405
LR  - 20111027
IS  - 0047-1887 (Print)
IS  - 0047-1887 (Linking)
VI  - 55
IP  - 3
DP  - 2001 Nov
TI  - [Application of immunohistochemistry for forensic pathological diagnosis: finding 
      of human brain in forensic autopsy].
PG  - 299-309
AB  - We researched the application of immunohistochemistry for the purpose of 
      establishing forensic pathological diagnoses. In the present study, we examined 
      the induction and expression of heat shock protein (HSP), oxygen regulated 
      protein (ORP), inducible nitric oxide synthase (iNOS), excitatory amino acid 
      transporter 2 (EAAT2) and apolipoprotein E (apo E) in the human brain using 
      forensic autopsy cases as our subjects. Hypoxic/ischemic brain damage. In cases 
      of longer survival and with a history of hypoxic attacks, the proteins HSP and 
      ORP were found in the parieto-occipital lobe and hippocampus. And we are able to 
      observe a weak stain for EAAT2 in almost all asphyxia deaths. Traumatic brain 
      injury (TBI). In traumatic brain injury (TBI), the prolonged induction of iNOS 
      was demonstrated in the neutrophils, microglia/macrophage, and vascular smooth 
      muscle cells in the traumatized brain. Apo E was identified with neurons in the 
      traumatized cortical hemisphere from only a two-hour survival case to long 
      survival cases. To the contrary, there was no positive apo E staining in the 
      contralateral cortical hemisphere at all. In one one-hour survival case, a weak 
      stain for EAAT2 was observed, but intensive expression of EAAT2 was observed from 
      brief to one-day survival cases. Sudden infant death (SID). Numerous 
      ferritin-positive cells were observed in the brain in the cases of pneumonia or 
      myocarditis that we examined. To the contrary, the numbers of ferritin-positive 
      cells were obviously decreased in the cases of sudden infant death syndrome 
      (SIDS). The transferrin-positive cells were in an inverse proportion to the 
      ferritin positive cells in each SIDS case. Also, numerous ORP-150 positive cells 
      were observed in the brain in cases of pneumonia and the SIDS group. In forensic 
      practice, immunohistochemical investigation of these proteins can be a great 
      value for diagnosing not only the cause of death but also the pathophysiological 
      changes and the victims past history.
FAU - Nakasono, I
AU  - Nakasono I
AD  - Department of Legal Medicine, Nagasaki University School of Medicine, Nagasaki 
      852-8523, Japan.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Nihon Hoigaku Zasshi
JT  - Nihon hoigaku zasshi = The Japanese journal of legal medicine
JID - 0413715
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Autopsy/standards
MH  - Brain/enzymology/*pathology
MH  - Brain Injuries/*pathology
MH  - *Forensic Medicine
MH  - Humans
MH  - Hypoxia-Ischemia, Brain/pathology
MH  - Immunohistochemistry
MH  - Infant
MH  - Nitric Oxide Synthase/analysis
MH  - Nitric Oxide Synthase Type II
MH  - Sudden Infant Death/pathology
RF  - 21
EDAT- 2002/03/22 10:00
MHDA- 2002/04/06 10:01
CRDT- 2002/03/22 10:00
PHST- 2002/03/22 10:00 [pubmed]
PHST- 2002/04/06 10:01 [medline]
PHST- 2002/03/22 10:00 [entrez]
PST - ppublish
SO  - Nihon Hoigaku Zasshi. 2001 Nov;55(3):299-309.