PMID- 11907230
OWN - NLM
STAT- MEDLINE
DCOM- 20020419
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 76
IP  - 8
DP  - 2002 Apr
TI  - Long-distance base pairing in flock house virus RNA1 regulates subgenomic RNA3 
      synthesis and RNA2 replication.
PG  - 3905-19
AB  - Replication of flock house virus (FHV) RNA1 and production of subgenomic RNA3 in 
      the yeast Saccharomyces cerevisiae provide a useful tool for the dissection of 
      FHV molecular biology and host-encoded functions involved in RNA replication. The 
      replication template activity of RNA1 can be separated from its coding potential 
      by supplying the RNA1-encoded replication factor protein A in trans. We 
      constructed a trans-replication system in yeast to examine cis-acting elements in 
      RNA1 that control RNA3 production, as well as RNA1 and RNA2 replication. Two cis 
      elements controlling RNA3 production were found. A proximal subgenomic control 
      element was located just upstream of the RNA3 start site (nucleotides [nt] 2282 
      to 2777). A short distal element also controlling RNA3 production (distal 
      subgenomic control element) was identified 1.5 kb upstream, at nt 1229 to 1239. 
      Base pairing between these distal and proximal elements was shown to be essential 
      for RNA3 production by covariation analysis and in vivo selection of 
      RNA3-expressing replicons from plasmid libraries containing random sequences in 
      the distal element. Two distinct RNA1 replication elements (RE) were mapped 
      within the 3' quarter of RNA1: the intRE (nt 2322 to 2501) and the 3'RE (nt 2735 
      to 3011). The 3'RE significantly overlaps the RNA3 region in RNA1, and this 
      information was applied to produce improved RNA3-based vectors for foreign-gene 
      expression. In addition, replication of an RNA2 derivative was dependent on RNA1 
      templates capable of forming the long-distance interaction that controls RNA3 
      production.
FAU - Lindenbach, Brett D
AU  - Lindenbach BD
AD  - Howard Hughes Medical Institute, University of Wisconsin-Madison, Madison, 
      Wisconsin 53706-1596, USA.
FAU - Sgro, Jean-Yves
AU  - Sgro JY
FAU - Ahlquist, Paul
AU  - Ahlquist P
LA  - eng
GR  - R01 GM035072/GM/NIGMS NIH HHS/United States
GR  - R37 GM035072/GM/NIGMS NIH HHS/United States
GR  - GM35072/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Viral)
SB  - IM
MH  - *Base Pairing
MH  - Base Sequence
MH  - *Gene Expression Regulation, Viral
MH  - Genetic Complementation Test
MH  - Genetic Vectors
MH  - Molecular Sequence Data
MH  - Nodaviridae/*genetics/metabolism
MH  - Plasmids/genetics
MH  - RNA Virus Infections/virology
MH  - RNA, Viral/*biosynthesis
MH  - Saccharomyces cerevisiae/genetics
MH  - *Virus Replication
PMC - PMC136111
EDAT- 2002/03/22 10:00
MHDA- 2002/04/20 10:01
PMCR- 2002/04/01
CRDT- 2002/03/22 10:00
PHST- 2002/03/22 10:00 [pubmed]
PHST- 2002/04/20 10:01 [medline]
PHST- 2002/03/22 10:00 [entrez]
PHST- 2002/04/01 00:00 [pmc-release]
AID - 2141 [pii]
AID - 10.1128/jvi.76.8.3905-3919.2002 [doi]
PST - ppublish
SO  - J Virol. 2002 Apr;76(8):3905-19. doi: 10.1128/jvi.76.8.3905-3919.2002.