PMID- 11907242
OWN - NLM
STAT- MEDLINE
DCOM- 20020419
LR  - 20220330
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 76
IP  - 8
DP  - 2002 Apr
TI  - A natural intergenotypic recombinant of hepatitis C virus identified in St. 
      Petersburg.
PG  - 4034-43
AB  - Hepatitis C virus (HCV) evolution is thought to proceed by mutations within the 
      six genotypes. Here, we report on a viable spontaneous HCV recombinant and we 
      show that recombination may play a role in the evolution of this virus. 
      Previously, 149 HCV strains from St. Petersburg had been subtyped by limited 
      sequencing within the NS5B region. In the present study, the core regions of 41 
      of these strains were sequenced to investigate the concordance of HCV genotyping 
      for these two genomic regions. Two phylogenetically related HCV strains were 
      found to belong to different subtypes, 2k and 1b, according to sequence analysis 
      of the 5' untranslated region (5'UTR)-core and the NS5B regions, respectively. By 
      sequencing of the E2-p7-NS2 region, the crossover point was mapped within the NS2 
      region, probably between positions 3175 and 3176 (according to the numbering 
      system for strain pj6CF). Sequencing of the 5'UTR-core regions of four other HCV 
      strains, phylogenetically related to the above-mentioned two strains (based on 
      analysis within the NS5B region), revealed that these four strains were also 
      recombinants. Since a nonrecombinant 2k strain was found in St. Petersburg, the 
      recombination may have taken place there around a decade ago. Since the frequency 
      of this recombinant is now high enough to allow the detection of the recombinant 
      in a fraction of the city's population, it seems to be actively spreading there. 
      The reported recombinant is tentatively designated RF1-2k/1b, in agreement with 
      the nomenclature used for HIV recombinants. Recombination between HCV genotypes 
      must now be considered in the classification, laboratory diagnosis, and treatment 
      of HCV infection.
FAU - Kalinina, Olga
AU  - Kalinina O
AD  - St. Petersburg Pasteur Institute, St. Petersburg 197101, Russia.
FAU - Norder, Helene
AU  - Norder H
FAU - Mukomolov, Sergey
AU  - Mukomolov S
FAU - Magnius, Lars O
AU  - Magnius LO
LA  - eng
SI  - GENBANK/AY070167
SI  - GENBANK/AY070168
SI  - GENBANK/AY070169
SI  - GENBANK/AY070170
SI  - GENBANK/AY070171
SI  - GENBANK/AY070172
SI  - GENBANK/AY070173
SI  - GENBANK/AY070174
SI  - GENBANK/AY070175
SI  - GENBANK/AY070176
SI  - GENBANK/AY070177
SI  - GENBANK/AY070178
SI  - GENBANK/AY070179
SI  - GENBANK/AY070180
SI  - GENBANK/AY070181
SI  - GENBANK/AY070182
SI  - GENBANK/AY070183
SI  - GENBANK/AY070184
SI  - GENBANK/AY070185
SI  - GENBANK/AY070186
SI  - GENBANK/AY070187
SI  - GENBANK/AY070188
SI  - GENBANK/AY070189
SI  - GENBANK/AY070190
SI  - GENBANK/AY070191
SI  - GENBANK/AY070192
SI  - GENBANK/AY070193
SI  - GENBANK/AY070194
SI  - GENBANK/AY070195
SI  - GENBANK/AY070196
SI  - GENBANK/AY070197
SI  - GENBANK/AY070198
SI  - GENBANK/AY070199
SI  - GENBANK/AY070200
SI  - GENBANK/AY070201
SI  - GENBANK/AY070202
SI  - GENBANK/AY070203
SI  - GENBANK/AY070204
SI  - GENBANK/AY070205
SI  - GENBANK/AY070206
SI  - GENBANK/AY070207
SI  - GENBANK/AY070208
SI  - GENBANK/AY070209
SI  - GENBANK/AY070210
SI  - GENBANK/AY070211
SI  - GENBANK/AY070212
SI  - GENBANK/AY070213
SI  - GENBANK/AY070214
SI  - GENBANK/AY070215
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 2.7.7.48 (NS-5 protein, hepatitis C virus)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Base Sequence
MH  - Genotype
MH  - Hepacivirus/*classification/*genetics/isolation & purification
MH  - Hepatitis C/*virology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - *Recombination, Genetic
MH  - Russia
MH  - Sequence Analysis, DNA
MH  - Viral Nonstructural Proteins/genetics
PMC - PMC136067
EDAT- 2002/03/22 10:00
MHDA- 2002/04/20 10:01
PMCR- 2002/04/01
CRDT- 2002/03/22 10:00
PHST- 2002/03/22 10:00 [pubmed]
PHST- 2002/04/20 10:01 [medline]
PHST- 2002/03/22 10:00 [entrez]
PHST- 2002/04/01 00:00 [pmc-release]
AID - 1492 [pii]
AID - 10.1128/jvi.76.8.4034-4043.2002 [doi]
PST - ppublish
SO  - J Virol. 2002 Apr;76(8):4034-43. doi: 10.1128/jvi.76.8.4034-4043.2002.