PMID- 11908658
OWN - NLM
STAT- MEDLINE
DCOM- 20021011
LR  - 20220309
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 45
IP  - 1
DP  - 2002 Feb
TI  - Identification and chromosomal organization of two rye genome-specific RAPD 
      products useful as introgression markers in wheat.
PG  - 157-64
AB  - Two rye genome-specific random amplified polymorphic DNA (RAPD) markers were 
      identified for detection of rye introgression in wheat. Both markers were 
      amplified in all of the tested materials that contained rye chromatin such as 
      rye, hexaploid triticale, wheat-rye addition lines, and wheat varieties with 
      1BL.1RS translocation. Two cloned markers, designated pSc10C and pSc20H, were 
      1012 bp and 1494 bp, respectively. Sequence analysis showed that both pSc10C and 
      pSc20H fragments were related to retrotransposons, ubiquitously distributed in 
      plant genomes. Using fluorescence in situ hybridization (FISH), probe pSc10C was 
      shown to hybridize predominantly to the pericentromeric regions of all rye 
      chromosomes, whereas probe pSc20H was dispersed throughout the rye genome except 
      at telomeric regions and nucleolar organizing regions. The FISH patterns showed 
      that the two markers should be useful to select or track all wheat-rye 
      translocation lines derived from the whole arms of rye chromosomes, as well as to 
      characterize the positions of the translocation breakpoints generated in the 
      proximal and distal regions of rye arms.
FAU - Ko, Jong-Min
AU  - Ko JM
AD  - National Yeongnam Agricultural Experiment Station, Milyang, Korea. 
      kojmin@rda.go.kr
FAU - Do, Geum-Sook
AU  - Do GS
FAU - Suh, Duck-Yong
AU  - Suh DY
FAU - Seo, Bong-Bo
AU  - Seo BB
FAU - Shin, Doo-Chull
AU  - Shin DC
FAU - Moon, Huhn-Pal
AU  - Moon HP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Base Sequence
MH  - Genetic Markers
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - *Random Amplified Polymorphic DNA Technique
MH  - Secale/*genetics
MH  - Sequence Analysis, DNA
MH  - Triticum/*genetics
EDAT- 2002/03/23 10:00
MHDA- 2002/10/12 04:00
CRDT- 2002/03/23 10:00
PHST- 2002/03/23 10:00 [pubmed]
PHST- 2002/10/12 04:00 [medline]
PHST- 2002/03/23 10:00 [entrez]
AID - 10.1139/g01-133 [doi]
PST - ppublish
SO  - Genome. 2002 Feb;45(1):157-64. doi: 10.1139/g01-133.