PMID- 11908659
OWN - NLM
STAT- MEDLINE
DCOM- 20021011
LR  - 20190916
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 45
IP  - 1
DP  - 2002 Feb
TI  - A microsatellite sequence from the rice blast fungus (Magnaporthe grisea) 
      distinguishes between the centromeres of Hordeum vulgare and H. bulbosum in 
      hybrid plants.
PG  - 165-74
AB  - A TC/AG-repeat microsatellite sequence derived from the rice blast fungus 
      (Magnaporthe grisea) hybridized to all of the centromeres of Hordeum vulgare 
      chromosomes, but hybridized faintly or not at all to the chromosomes of Hordeum 
      bulbosum. Using this H. vulgare centromere-specific probe, the chromosomes of 
      four F1 hybrids between H. vulgare and H. bulbosum were analyzed. The chromosome 
      constitution in the root tips of the hybrids was mosaic, i.e., 7 (7v, H. vulgare) 
      and 14 (7v + 7b H. bulbosum), or 14 (7v + 7b) and 27 (14v + 13b), or 7 (7v), 14 
      (7v + 7b), and 27 (14v + 13b). The 27-chromosome tetraploid hybrid cells were 
      revealed to have the NOR (nucleolus organizer region) bearing chromosome of H. 
      bulbosum in a hemizygous state, which might indicate some role for this 
      chromosome in the chromosome instability of the hybrid condition. The chromosomal 
      distribution showed that the chromosomes of H. vulgare were concentric and 
      chromosomes of H. bulbosum were peripheral in the mitotic squash. This non-random 
      chromosome distribution and the centromere-specific repeated DNA differences in 
      the two species were discussed in relation to H. bulbosum chromosome elimination. 
      Meiotic chromosome analyses revealed a high frequency of homoeologous chromosome 
      pairing in early prophase. However, this chromosome pairing did not persist until 
      later meiotic stages and many univalents and chromosome fragments resulted. These 
      were revealed to be H. bulbosum by fluorescence in situ hybridization (FISH) 
      analysis with the H. vulgare centromere-specific probe. Because the chromosome 
      segregation of H. vulgare and H. bulbosum chromosomes at anaphase I of meiosis 
      was random, the possibility for obtaining chromosome substitution lines in 
      diploid barley from the diploid hybrid was discussed.
FAU - Kim, N S
AU  - Kim NS
AD  - Division of Applied Plant Sciences, Kangwon National University, Chunchon, Korea.
FAU - Armstrong, K C
AU  - Armstrong KC
FAU - Fedak, G
AU  - Fedak G
FAU - Ho, K
AU  - Ho K
FAU - Park, N I
AU  - Park NI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Fungal)
SB  - IM
MH  - Base Sequence
MH  - Centromere/*genetics
MH  - Chromosomes
MH  - DNA, Fungal
MH  - Hordeum/classification/*genetics/microbiology
MH  - In Situ Hybridization
MH  - Magnaporthe/*genetics
MH  - Meiosis
MH  - *Microsatellite Repeats
MH  - Molecular Sequence Data
EDAT- 2002/03/23 10:00
MHDA- 2002/10/12 04:00
CRDT- 2002/03/23 10:00
PHST- 2002/03/23 10:00 [pubmed]
PHST- 2002/10/12 04:00 [medline]
PHST- 2002/03/23 10:00 [entrez]
AID - 10.1139/g01-129 [doi]
PST - ppublish
SO  - Genome. 2002 Feb;45(1):165-74. doi: 10.1139/g01-129.