PMID- 11908670
OWN - NLM
STAT- MEDLINE
DCOM- 20021011
LR  - 20190916
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 45
IP  - 1
DP  - 2002 Feb
TI  - S1 satellite DNA as a taxonomic marker in brown frogs: molecular evidence that 
      Rana graeca graeca and Rana graeca italica are different species.
PG  - 63-70
AB  - The brown frog Rana graeca was believed to be present in two areas, the Balkan 
      Peninsula and the Italian Apennines. We have characterised the S1 satellite DNA 
      family from Rana graeca graeca and compared it with that of Rana graeca italica. 
      On Southern blots, the patterns of S1 satellite DNA bands are very different 
      between Italian and Greek specimens, but homogeneous among various populations of 
      the same taxon. The satellite DNA from the Greek taxon contains two repetitive 
      units (S1a (494 bp) and S1b (363 bp)) that could be sequenced after amplification 
      from genomic DNA to directly yield their consensus sequences in each genome. 
      These consensus sequences were very similar among the Greek populations, but 
      differed either in sequence (in S1a) or in both size and sequence (in S1b) from 
      the corresponding repeats of the Italian taxon. A mechanism of concerted 
      evolution is likely responsible for the high homogeneity of S1a and S1b repeat 
      sequences within each genome and species. The genomic content of S1 satellite DNA 
      was lower in the Greek than in the Italian populations (0.5 vs. 1.9%) and 
      fluorescence in situ hybridization (FISH) analysis showed the S1 satellite on 
      only 4 chromosome pairs in the Greek taxon and on all 13 chromosome pairs in the 
      Italian taxon. The completely different structure and genomic organization of the 
      S1 satellite DNA indicate that the Greek and Italian taxa are distinct species: 
      R. graeca and R. italica.
FAU - Picariello, Orfeo
AU  - Picariello O
AD  - Dipartimento di Zoologia, Universita Federico II, Naples, Italy.
FAU - Feliciello, Isidoro
AU  - Feliciello I
FAU - Bellinello, Renato
AU  - Bellinello R
FAU - Chinali, Gianni
AU  - Chinali G
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Satellite)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosome Mapping
MH  - Conserved Sequence
MH  - *DNA, Satellite
MH  - Evolution, Molecular
MH  - *Genetic Markers
MH  - Molecular Sequence Data
MH  - Ranidae/classification/*genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 2002/03/23 10:00
MHDA- 2002/10/12 04:00
CRDT- 2002/03/23 10:00
PHST- 2002/03/23 10:00 [pubmed]
PHST- 2002/10/12 04:00 [medline]
PHST- 2002/03/23 10:00 [entrez]
AID - 10.1139/g01-125 [doi]
PST - ppublish
SO  - Genome. 2002 Feb;45(1):63-70. doi: 10.1139/g01-125.