PMID- 11909860
OWN - NLM
STAT- MEDLINE
DCOM- 20020702
LR  - 20210206
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 22
DP  - 2002 May 31
TI  - The viral transactivator E1A regulates the mouse mammary tumor virus promoter in 
      an isoform- and chromatin-specific manner.
PG  - 19847-54
AB  - Proteins encoded by the adenovirus E1A gene regulate both cellular and viral 
      genes to mediate effects on cell cycle, differentiation, and cell growth control. 
      We have identified the mouse mammary tumor virus (MMTV) promoter as a target of 
      E1A action and investigated the role nucleoprotein structure plays in its 
      response to E1A. Both 12 and 13 S forms target the MMTV promoter when it has a 
      disorganized and accessible chromatin configuration. However, whereas the 13 S 
      form is stimulatory, the 12 S form is repressive. When the MMTV promoter adopts 
      an organized and repressed chromatin structure, it is targeted only by the 13 S 
      form, which stimulates it. Although evidence indicates that E1A interacts with 
      the SWI/SNF remodeling complex, E1A had no effect on chromatin remodeling at the 
      MMTV promoter in organized chromatin. Analysis of E1A mutants showed that 
      stimulation of the MMTV promoter is mediated solely through conserved region 3 
      and does not require interaction with Rb, p300/CBP-associated factor, or 
      CBP/p300. Imaging analysis showed that E1A colocalizes with MMTV sequences in 
      vivo, suggesting that it functions directly at the promoter. These results 
      indicate that E1A stimulates the MMTV promoter in a fashion independent of 
      chromatin conformation and through a direct mechanism involving interaction with 
      the basal transcription machinery.
FAU - Soeth, Edlyn
AU  - Soeth E
AD  - Signal Transduction Group, Laboratory of Receptor Biology and Gene Expression, 
      Center for Cancer Research, NCI, National Institutes of Health, Bethesda, 
      Maryland 20892, USA.
FAU - Thurber, Denise B
AU  - Thurber DB
FAU - Smith, Catharine L
AU  - Smith CL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020321
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adenovirus E1A Proteins)
RN  - 0 (Chromatin)
RN  - 0 (Protein Isoforms)
RN  - 63231-63-0 (RNA)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Adenovirus E1A Proteins/*metabolism/*physiology
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Chromatin/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Genetic Vectors
MH  - Luciferases/metabolism
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Mutation
MH  - Plasmids/metabolism
MH  - *Promoter Regions, Genetic
MH  - Protein Binding
MH  - Protein Isoforms
MH  - RNA/metabolism
MH  - Transcription, Genetic
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2002/03/23 10:00
MHDA- 2002/07/03 10:01
CRDT- 2002/03/23 10:00
PHST- 2002/03/23 10:00 [pubmed]
PHST- 2002/07/03 10:01 [medline]
PHST- 2002/03/23 10:00 [entrez]
AID - S0021-9258(20)85053-4 [pii]
AID - 10.1074/jbc.M200629200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 May 31;277(22):19847-54. doi: 10.1074/jbc.M200629200. Epub 2002 
      Mar 21.