PMID- 11916498
OWN - NLM
STAT- MEDLINE
DCOM- 20020523
LR  - 20181023
IS  - 1522-8517 (Print)
IS  - 1523-5866 (Electronic)
IS  - 1522-8517 (Linking)
VI  - 4
IP  - 2
DP  - 2002 Apr
TI  - Comprehensive molecular cytogenetic investigation of chromosomal abnormalities in 
      human medulloblastoma cell lines and xenograft.
PG  - 75-85
LID - 10.1093/neuonc/4.2.75 [doi]
AB  - Cell lines and xenografts derived from medulloblastomas are useful tools to 
      investigate the chromosomal changes in these tumors. Here we used G-banding, 
      fluorescence in situ hybridization (FISH), spectral karyotyping (SKY), and 
      comparative genomic hybridization to study 4 medulloblastoma cell lines and 1 
      xenograft. Cell line D-425 Med had a relatively simple karyotype, with a terminal 
      deletion of 10q and amplification of MYC in double-minutes (dmins). FISH 
      demonstrated that an apparent isochromosome (17q) by routine karyotyping was 
      actually an unbalanced translocation between 2 copies of chromosome 17. Cell line 
      D-556 Med also had a simple near-diploid stemline with an unbalanced 1;13 
      translocation resulting in a gain of 1q, an isochromosome (17q), and dmins. These 
      findings were initially described using routine G-banded preparations, and FISH 
      showed that the dmins were an amplification of MYC and the i(17q) was an 
      isodicentric 17q chromosome. The other finding was confirmed by FISH, SKY, and 
      comparative genomic hybridization. Cell lines D-721 Med and D-581 Med had complex 
      karyotypic patterns that could be completely characterized only when FISH and SKY 
      were used. Xenograft D-690 Med also had a complex pattern that FISH and SKY were 
      helpful in completely elucidating. Interestingly, balanced reciprocal 
      translocations were seen as well as complicated unbalanced translocations and 
      marker chromosomes. Comparative genomic hybridization demonstrated only a 
      deletion of 10q22-10q24, supporting the idea that despite the complexity of the 
      chromosomal rearrangements, minimal alterations in the overall chromosomal 
      content had occurred. This study demonstrates that routine cytogenetic 
      preparations are adequate to describe chromosomal abnormalities in occasional 
      medulloblastoma samples, but a broader spectrum of molecular cytogenetic methods 
      is required to completely analyze most of these tumor samples.
FAU - Aldosari, Naji
AU  - Aldosari N
AD  - Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA.
FAU - Wiltshire, Rodney N
AU  - Wiltshire RN
FAU - Dutra, Amalia
AU  - Dutra A
FAU - Schrock, Evelin
AU  - Schrock E
FAU - McLendon, Roger E
AU  - McLendon RE
FAU - Friedman, Henry S
AU  - Friedman HS
FAU - Bigner, Darell D
AU  - Bigner DD
FAU - Bigner, Sandra H
AU  - Bigner SH
LA  - eng
GR  - CA-68119/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Neuro Oncol
JT  - Neuro-oncology
JID - 100887420
SB  - IM
MH  - Adolescent
MH  - Cerebellar Neoplasms/*genetics
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Medulloblastoma/*genetics
MH  - Nucleic Acid Hybridization
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured
PMC - PMC1920654
EDAT- 2002/03/28 10:00
MHDA- 2002/05/25 10:01
PMCR- 2003/04/01
CRDT- 2002/03/28 10:00
PHST- 2001/07/13 00:00 [received]
PHST- 2001/10/08 00:00 [accepted]
PHST- 2002/03/28 10:00 [pubmed]
PHST- 2002/05/25 10:01 [medline]
PHST- 2002/03/28 10:00 [entrez]
PHST- 2003/04/01 00:00 [pmc-release]
AID - 10.1093/neuonc/4.2.75 [doi]
PST - ppublish
SO  - Neuro Oncol. 2002 Apr;4(2):75-85. doi: 10.1093/neuonc/4.2.75.